A bright and responsive europium probe for determination of pH change within the endoplasmic reticulum of living cells.

McMahon, Brian K.; Pal, Robert; Parker, David

Abstract

A ratiometric EuIII complex has been developed that localises selectively within the endoplasmic reticulum of living cells. Careful calibration, using a time-gated spectral imaging microscope, allows the intensity ratio of emission bands and the variation of excited state lifetime to be used for pH determination, with a pKa of 7.15.

Citation

McMahon, B. K., Pal, R., & Parker, D. (2013). A bright and responsive europium probe for determination of pH change within the endoplasmic reticulum of living cells. Chemical Communications, 49(47), 5363-5365. https://doi.org/10.1039/c3cc42308e

• Journal Article Type: Article
• Acceptance Date: Apr 30, 2013
• Online Publication Date: May 1, 2013
• Publication Date: 2013-06
• Deposit Date: Mar 17, 2017
• Journal: Chemical Communications
• Print ISSN: 1359-7345
• Electronic ISSN: 1364-548X
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 49
• Issue: 47
• Pages: 5363-5365
• DOI: https://doi.org/10.1039/c3cc42308e