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Structural Control of Cell Permeability with Highly Emissive Europium(III) Complexes Permits Different Microscopy Applications

Starck, Matthieu; Pal, Robert; Parker, David

Authors

David Parker



Abstract

Four anionic europium complexes are described based on triazacyclononane tris-carboxylate or phosphinate ligands. In each case, the three sensitising chromophores comprise a substituted aryl–alkynyl pyridine group, with complex brightness in water falling in the range 4 to 23 mM−1 cm−1. para-Substitution of the aryl ring with carboxymethyl groups gives complexes that are taken into cells, stain the lysosomes selectively and unexpectedly permit lifetime measurements of lysosomal pH. In contrast, the introduction of sulfonate groups inhibits cell uptake enabling the Eu complex to be used as an extracellular donor for FRET applications at the membrane surface. Using time-gated FRET microscopy, the cell membrane structure was highlighted, in which Cell Mask Deep Red was used as a membrane- localized FRET acceptor.

Citation

Starck, M., Pal, R., & Parker, D. (2016). Structural Control of Cell Permeability with Highly Emissive Europium(III) Complexes Permits Different Microscopy Applications. Chemistry - A European Journal, 22(2), 570-580. https://doi.org/10.1002/chem.201504103

Journal Article Type Article
Online Publication Date Jan 11, 2016
Publication Date 2016-01
Deposit Date Mar 17, 2017
Journal Chemistry - A European Journal
Print ISSN 0947-6539
Electronic ISSN 1521-3765
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 22
Issue 2
Pages 570-580
DOI https://doi.org/10.1002/chem.201504103
Public URL https://durham-repository.worktribe.com/output/1390753