Dr Matthieu Starck matthieu.starck@durham.ac.uk
Postdoctoral Research Associate
Dr Matthieu Starck matthieu.starck@durham.ac.uk
Postdoctoral Research Associate
Professor Robert Pal robert.pal@durham.ac.uk
Professor
David Parker
Four anionic europium complexes are described based on triazacyclononane tris-carboxylate or phosphinate ligands. In each case, the three sensitising chromophores comprise a substituted aryl–alkynyl pyridine group, with complex brightness in water falling in the range 4 to 23 mM−1 cm−1. para-Substitution of the aryl ring with carboxymethyl groups gives complexes that are taken into cells, stain the lysosomes selectively and unexpectedly permit lifetime measurements of lysosomal pH. In contrast, the introduction of sulfonate groups inhibits cell uptake enabling the Eu complex to be used as an extracellular donor for FRET applications at the membrane surface. Using time-gated FRET microscopy, the cell membrane structure was highlighted, in which Cell Mask Deep Red was used as a membrane- localized FRET acceptor.
Starck, M., Pal, R., & Parker, D. (2016). Structural Control of Cell Permeability with Highly Emissive Europium(III) Complexes Permits Different Microscopy Applications. Chemistry - A European Journal, 22(2), 570-580. https://doi.org/10.1002/chem.201504103
Journal Article Type | Article |
---|---|
Online Publication Date | Jan 11, 2016 |
Publication Date | 2016-01 |
Deposit Date | Mar 17, 2017 |
Journal | Chemistry - A European Journal |
Print ISSN | 0947-6539 |
Electronic ISSN | 1521-3765 |
Publisher | Wiley |
Peer Reviewed | Peer Reviewed |
Volume | 22 |
Issue | 2 |
Pages | 570-580 |
DOI | https://doi.org/10.1002/chem.201504103 |
Public URL | https://durham-repository.worktribe.com/output/1390753 |
Hierarchical self-assembly in an RNA-based coordination polymer hydrogel
(2023)
Journal Article
The Pink Box: Exclusive Homochiral Aromatic Stacking in a Bis-perylene Diimide Macrocycle
(2022)
Journal Article
About Durham Research Online (DRO)
Administrator e-mail: dro.admin@durham.ac.uk
This application uses the following open-source libraries:
Apache License Version 2.0 (http://www.apache.org/licenses/)
Apache License Version 2.0 (http://www.apache.org/licenses/)
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2025
Advanced Search