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Cyclin-dependent kinase activity enhances phosphatidylcholine biosynthesis in Arabidopsis by repressing phosphatidic acid phosphohydrolase activity

Craddock, Christian P.; Adams, Nicolette; Kroon, Johan T.M.; Bryant, Fiona M.; Hussey, Patrick J.; Kurup, Smita; Eastmond, Peter J.

Cyclin-dependent kinase activity enhances phosphatidylcholine biosynthesis in Arabidopsis by repressing phosphatidic acid phosphohydrolase activity Thumbnail


Authors

Christian P. Craddock

Nicolette Adams

Fiona M. Bryant

Smita Kurup

Peter J. Eastmond



Abstract

Coordination of endomembrane biogenesis with cell cycle progression is considered to be important in maintaining cell function during growth and development. We previously showed that disruption of PHOSPHATIDIC ACID PHOSPHOHYDROLASE (PAH) activity in Arabidopsis thaliana stimulates biosynthesis of the major phospholipid phosphatidylcholine (PC) and causes expansion of the endoplasmic reticulum. Here we show that PC biosynthesis is repressed by disruption of the core cell cycle regulator CYCLIN-DEPENDENT KINASE A;1 (CDKA;1) and that this repression is reliant on PAH. Furthermore, we show that CDKs phosphorylate PAH1 at serine 162, which reduces both its activity and membrane association. Expression of a CDK-insensitive version of PAH1 with a serine 162 to alanine substitution represses PC biosynthesis and also reduces the rate of cell division in early leaf development. Together our findings reveal a physiologically important mechanism that couples the rate of phospholipid biosynthesis and endomembrane biogenesis to cell cycle progression in Arabidopsis.

Journal Article Type Article
Acceptance Date Aug 22, 2016
Online Publication Date Dec 1, 2016
Publication Date Sep 6, 2016
Deposit Date Sep 19, 2016
Publicly Available Date Sep 19, 2016
Journal Plant Journal
Print ISSN 0960-7412
Electronic ISSN 1365-313X
Publisher Wiley
Peer Reviewed Peer Reviewed
DOI https://doi.org/10.1111/tpj.13321
Public URL https://durham-repository.worktribe.com/output/1376371

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Accepted Journal Article (861 Kb)
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Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/

Copyright Statement
This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi: 10.1111/tpj.13321






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