Personal protection of permethrin-treated clothing against Aedes aegypti, the vector of dengue and Zika virus, in the laboratory

Orsborne, J.; DeRaedt Banks, S.; Hendy, A.; Gezan, S.A.; Kaur, H.; Wilder-Smith, A.; Lindsay, S.W.; Logan, J.G.

Abstract

Background The dengue and Zika viruses are primarily transmitted by Aedes aegypti mosquitoes, which are most active during day light hours and feed both in and outside of the household. Personal protection technologies such as insecticide-treated clothing could provide individual protection. Here we assessed the efficacy of permethrin-treated clothing on personal protection in the laboratory. Methods The effect of washing on treated clothing, skin coverage and protection against resistant and susceptible Ae. aegypti was assessed using modified WHO arm-in-cage assays. Coverage was further assessed using free-flight room tests to investigate the protective efficacy of unwashed factory-dipped permethrin-treated clothing. Clothing was worn as full coverage (long sleeves and trousers) and partial coverage (short sleeves and shorts). Residual permethrin on the skin and its effect on mosquitoes was measured using modified WHO cone assays and quantified using high-pressure liquid chromatography (HPLC) analysis. Results In the arm-in-cage assays, unwashed clothing reduced landing by 58.9% (95% CI 49.2–66.9) and biting by 28.5% (95% CI 22.5–34.0), but reduced to 18.5% (95% CI 14.7–22.3) and 11.1% (95% CI 8.5–13.8) respectively after 10 washes. Landing and biting for resistant and susceptible strains was not significantly different (p<0.05). In free-flight room tests, full coverage treated clothing reduced landing by 24.3% (95% CI 17.4–31.7) and biting by 91% (95% CI 82.2–95.9) with partial coverage reducing landing and biting by 26.4% (95% CI 20.3–31.2) and 49.3% (95% CI 42.1–59.1) respectively with coverage type having no significant difference on landing (p<0.05). Residual permethrin was present on the skin in low amounts (0.0041mg/cm2), but still produced a KD of >80% one hour after wearing treated clothing. Conclusion Whilst partially covering the body with permethrin-treated clothing provided some protection against biting, wearing treated clothing with long sleeves and trousers provided the highest form of protection. Washing treated clothing dramatically reduced protection provided. Permethrin-treated clothing could provide protection to individuals from Ae. aegypti that show permethrin resistance. Additionally, it could continue to provide protection even after the clothing has been worn. Field trials are urgently needed to determine whether clothing can protect against dengue and Zika.

Citation

Orsborne, J., DeRaedt Banks, S., Hendy, A., Gezan, S., Kaur, H., Wilder-Smith, A., …Logan, J. (2016). Personal protection of permethrin-treated clothing against Aedes aegypti, the vector of dengue and Zika virus, in the laboratory. PLoS ONE, 11(5), Article e0152805. https://doi.org/10.1371/journal.pone.0152805

• Journal Article Type: Article
• Acceptance Date: Mar 18, 2016
• Online Publication Date: May 17, 2016
• Publication Date: May 17, 2016
• Deposit Date: Dec 9, 2016
• Publicly Available Date: Dec 9, 2016
• Journal: PLoS ONE
• Publisher: Public Library of Science
• Peer Reviewed: Peer Reviewed
• Volume: 11
• Issue: 5
• Article Number: e0152805
• DOI: https://doi.org/10.1371/journal.pone.0152805
• Public URL: https://durham-repository.worktribe.com/output/1368668