Skip to main content

Research Repository

Advanced Search

Substrate-led cholesterol extraction from supported lipid membranes

Miller, Ethan; Voitchovsky, Kislon; Staykova, Margarita

Substrate-led cholesterol extraction from supported lipid membranes Thumbnail


Ethan Miller


The lipid membrane is a principal building block in biology, technology and industry, where it often occurs supported by other hydrophilic structures. Interactions with the support can affect the physical behavior of the membrane from the local organization and diffusion of lipids and proteins, to phase transitions, and the local mechanical properties. In this study we show that supporting substrates textured with nanoscale hydrophilic and hydrophobic domains can modify the membrane's chemical composition by selectively extracting cholesterol molecules without affecting the remaining phospholipids. Using polydimethylsiloxane (PDMS) substrates with various degrees of plasma oxidation, we are able to trigger dramatic changes in the membrane morphology and biophysical properties, and relate them to the amount of extracted cholesterol. We also show that it is possible to control the cholesterol extraction through mechanical extension of the flexible PDMS support. Given the ubiquity of bio-substrates with textured surface properties and the wide use of PDMS we expect that our results will have implications not only in biological and chemical sciences but also in nanotechnologies such as organ on a chip technologies, biosensors, and stretchable bio-electronics.


Miller, E., Voitchovsky, K., & Staykova, M. (2018). Substrate-led cholesterol extraction from supported lipid membranes. Nanoscale, 10(34), 16332-16342.

Journal Article Type Article
Acceptance Date Aug 7, 2018
Online Publication Date Aug 22, 2018
Publication Date Sep 14, 2018
Deposit Date Aug 8, 2018
Publicly Available Date Aug 31, 2018
Journal Nanoscale
Print ISSN 2040-3364
Electronic ISSN 2040-3372
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 10
Issue 34
Pages 16332-16342
Public URL


You might also like

Downloadable Citations