Enhancing multi-spot structured illumination microscopy with fluorescence difference

Ward, Edward N.; Torkelsen, Frida H.; Pal, Robert

Abstract

Structured illumination microscopy is a super-resolution technique used extensively in biological research. However, this technique is limited in the maximum possible resolution increase. Here we report the results of simulations of a novel enhanced multi-spot structured illumination technique. This method combines the super-resolution technique of difference microscopy with structured illumination deconvolution. Initial results give at minimum a 1.4-fold increase in resolution over conventional structured illumination in a low-noise environment. This new technique also has the potential to be expanded to further enhance axial resolution with three-dimensional difference microscopy. The requirement for precise pattern determination in this technique also led to the development of a new pattern estimation algorithm which proved more efficient and reliable than other methods tested.

Citation

Ward, E. N., Torkelsen, F. H., & Pal, R. (2018). Enhancing multi-spot structured illumination microscopy with fluorescence difference. Royal Society Open Science, 5(3), Article 171336. https://doi.org/10.1098/rsos.171336

• Journal Article Type: Article
• Acceptance Date: Feb 9, 2018
• Online Publication Date: Mar 14, 2018
• Publication Date: Mar 14, 2018
• Deposit Date: Mar 29, 2018
• Publicly Available Date: Mar 29, 2018
• Journal: Royal Society Open Science
• Publisher: The Royal Society
• Peer Reviewed: Peer Reviewed
• Volume: 5
• Issue: 3
• Article Number: 171336
• DOI: https://doi.org/10.1098/rsos.171336