Edward N. Ward
Enhancing multi-spot structured illumination microscopy with fluorescence difference
Ward, Edward N.; Torkelsen, Frida H.; Pal, Robert
Abstract
Structured illumination microscopy is a super-resolution technique used extensively in biological research. However, this technique is limited in the maximum possible resolution increase. Here we report the results of simulations of a novel enhanced multi-spot structured illumination technique. This method combines the super-resolution technique of difference microscopy with structured illumination deconvolution. Initial results give at minimum a 1.4-fold increase in resolution over conventional structured illumination in a low-noise environment. This new technique also has the potential to be expanded to further enhance axial resolution with three-dimensional difference microscopy. The requirement for precise pattern determination in this technique also led to the development of a new pattern estimation algorithm which proved more efficient and reliable than other methods tested.
Citation
Ward, E. N., Torkelsen, F. H., & Pal, R. (2018). Enhancing multi-spot structured illumination microscopy with fluorescence difference. Royal Society Open Science, 5(3), Article 171336. https://doi.org/10.1098/rsos.171336
Journal Article Type | Article |
---|---|
Acceptance Date | Feb 9, 2018 |
Online Publication Date | Mar 14, 2018 |
Publication Date | Mar 14, 2018 |
Deposit Date | Mar 29, 2018 |
Publicly Available Date | Mar 29, 2018 |
Journal | Royal Society Open Science |
Publisher | The Royal Society |
Peer Reviewed | Peer Reviewed |
Volume | 5 |
Issue | 3 |
Article Number | 171336 |
DOI | https://doi.org/10.1098/rsos.171336 |
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Copyright Statement
© 2018 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
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