E.C. Pinneh
Expression levels of inositol phosphorylceramide synthase modulate plant responses to biotic and abiotic stress in Arabidopsis thaliana
Pinneh, E.C.; Stoppel, R.; Knight, H.; Knight, M.R.; Steel, P.G.; Denny, P.W.
Authors
R. Stoppel
Professor Heather Knight p.h.knight@durham.ac.uk
Professor
Professor Marc Knight m.r.knight@durham.ac.uk
Professor
P.G. Steel
Professor Paul Denny p.w.denny@durham.ac.uk
Professor
Abstract
This research was undertaken to investigate the global role of the plant inositol phosphorylceramide synthase (IPCS), a non-mammalian enzyme previously shown to be associated with the pathogen response. RNA-Seq analyses demonstrated that over-expression of inositol phosphorylceramide synthase isoforms AtIPCS1, 2 or 3 in Arabidopsis thaliana resulted in the down-regulation of genes involved in plant response to pathogens. In addition, genes associated with the abiotic stress response to salinity, cold and drought were found to be similarly down-regulated. Detailed analyses of transgenic lines over-expressing AtIPCS1-3 at various levels revealed that the degree of down-regulation is specifically correlated with the level of IPCS expression. Singular enrichment analysis of these down-regulated genes showed that AtIPCS1-3 expression affects biological signaling pathways involved in plant response to biotic and abiotic stress. The up-regulation of genes involved in photosynthesis and lipid localization was also observed in the over-expressing lines.
Citation
Pinneh, E., Stoppel, R., Knight, H., Knight, M., Steel, P., & Denny, P. (2019). Expression levels of inositol phosphorylceramide synthase modulate plant responses to biotic and abiotic stress in Arabidopsis thaliana. PLoS ONE, 14(5), Article e0217087. https://doi.org/10.1371/journal.pone.0217087
Journal Article Type | Article |
---|---|
Acceptance Date | Apr 30, 2019 |
Online Publication Date | May 23, 2019 |
Publication Date | May 23, 2019 |
Deposit Date | Sep 13, 2018 |
Publicly Available Date | May 24, 2019 |
Journal | PLoS ONE |
Electronic ISSN | 1932-6203 |
Publisher | Public Library of Science |
Peer Reviewed | Peer Reviewed |
Volume | 14 |
Issue | 5 |
Article Number | e0217087 |
DOI | https://doi.org/10.1371/journal.pone.0217087 |
Public URL | https://durham-repository.worktribe.com/output/1314812 |
Files
Published Journal Article
(2.7 Mb)
PDF
Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/
Copyright Statement
Copyright: © 2019 Pinneh et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
You might also like
Low-temperature and circadian signals are integrated by the sigma factor SIG5
(2023)
Journal Article
Impact of Cell‐wall Structure and Composition on Plant Freezing Tolerance
(2020)
Book Chapter
Downloadable Citations
About Durham Research Online (DRO)
Administrator e-mail: dro.admin@durham.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2024
Advanced Search