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Expression levels of inositol phosphorylceramide synthase modulate plant responses to biotic and abiotic stress in Arabidopsis thaliana

Pinneh, E.C.; Stoppel, R.; Knight, H.; Knight, M.R.; Steel, P.G.; Denny, P.W.

Expression levels of inositol phosphorylceramide synthase modulate plant responses to biotic and abiotic stress in Arabidopsis thaliana Thumbnail


Authors

E.C. Pinneh

R. Stoppel

P.G. Steel



Abstract

This research was undertaken to investigate the global role of the plant inositol phosphorylceramide synthase (IPCS), a non-mammalian enzyme previously shown to be associated with the pathogen response. RNA-Seq analyses demonstrated that over-expression of inositol phosphorylceramide synthase isoforms AtIPCS1, 2 or 3 in Arabidopsis thaliana resulted in the down-regulation of genes involved in plant response to pathogens. In addition, genes associated with the abiotic stress response to salinity, cold and drought were found to be similarly down-regulated. Detailed analyses of transgenic lines over-expressing AtIPCS1-3 at various levels revealed that the degree of down-regulation is specifically correlated with the level of IPCS expression. Singular enrichment analysis of these down-regulated genes showed that AtIPCS1-3 expression affects biological signaling pathways involved in plant response to biotic and abiotic stress. The up-regulation of genes involved in photosynthesis and lipid localization was also observed in the over-expressing lines.

Citation

Pinneh, E., Stoppel, R., Knight, H., Knight, M., Steel, P., & Denny, P. (2019). Expression levels of inositol phosphorylceramide synthase modulate plant responses to biotic and abiotic stress in Arabidopsis thaliana. PLoS ONE, 14(5), Article e0217087. https://doi.org/10.1371/journal.pone.0217087

Journal Article Type Article
Acceptance Date Apr 30, 2019
Online Publication Date May 23, 2019
Publication Date May 23, 2019
Deposit Date Sep 13, 2018
Publicly Available Date May 24, 2019
Journal PLoS ONE
Electronic ISSN 1932-6203
Publisher Public Library of Science
Peer Reviewed Peer Reviewed
Volume 14
Issue 5
Article Number e0217087
DOI https://doi.org/10.1371/journal.pone.0217087
Public URL https://durham-repository.worktribe.com/output/1314812

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Published Journal Article (2.7 Mb)
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Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/

Copyright Statement
Copyright: © 2019 Pinneh et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.






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