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Protein-Ligand Fishing in planta for Biologically Active Natural Products Using Glutathione Transferases

Dixon, David P.; Edwards, Robert

Protein-Ligand Fishing in planta for Biologically Active Natural Products Using Glutathione Transferases Thumbnail


David P. Dixon

Robert Edwards


Screening for natural products which bind to proteins in planta has been used to identify ligands of the plant-specific glutathione transferase (GST) tau (U) and phi (F) classes, that are present in large gene families in crops and weeds, but have largely undefined functions. When expressed as recombinant proteins in Escherichia coli these proteins have been found to tightly bind a diverse range of natural product ligands, with fatty acid-and porphyrinogen-derivatives associated with GSTUs and a range of heterocyclic compounds with GSTFs. With an interest in detecting the natural binding partners of these proteins in planta, we have expressed the two best characterized GSTs from Arabidopsis thaliana (At), AtGSTF2 and AtGSTU19, as Strep-tagged fusion proteins in planta. Following transient and stable expression in Nicotiana and Arabidopsis, respectively, the GSTs were recovered using Strep-Tactin affinity chromatography and the bound ligands desorbed and characterized by LC-MS. AtGSTF2 predominantly bound phenolic derivatives including S-glutathionylated lignanamides and methylated variants of the flavonols kaempferol and quercetin. AtGSTU19 captured glutathionylated conjugates of oxylipins, indoles, and lignanamides. Whereas the flavonols and oxylipins appeared to be authentic in vivo ligands, the glutathione conjugates of the lignanamides and indoles were artifacts formed during extraction. When tested for their binding characteristics, the previously undescribed indole conjugates were found to be particularly potent inhibitors of AtGSTU19. Such ligand fishing has the potential to both give new insight into protein function in planta as well as identifying novel classes of natural product inhibitors of enzymes of biotechnological interest such as GSTs.


Dixon, D. P., & Edwards, R. (2018). Protein-Ligand Fishing in planta for Biologically Active Natural Products Using Glutathione Transferases. Frontiers in Plant Science, 9, Article 1659.

Journal Article Type Article
Acceptance Date Oct 25, 2018
Online Publication Date Nov 15, 2018
Publication Date Nov 15, 2018
Deposit Date Nov 29, 2018
Publicly Available Date Nov 29, 2018
Journal Frontiers in Plant Science
Publisher Frontiers Media
Peer Reviewed Peer Reviewed
Volume 9
Article Number 1659


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Copyright Statement
© 2018 Dixon and Edwards. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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