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The Influence of Cholesterol on Melittin Lipidation in Neutral Membranes

Britt, Hannah M.; Mosely, Jackie A.; Sanderson, John M.

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Authors

Hannah M. Britt

Jackie A. Mosely



Abstract

The effects of cholesterol on the process of intrinsic lipidation, whereby an acyl chain is transferred from a lipid as donor to a membrane-associated acceptor molecule, have been explored using melittin as the acceptor. Membranes comprising lipids with saturated acyl chains (1,2-dipalmitoyl sn-glycero-3-phosphocholine, DPPC; 1,2-dimyristoyl sn-glycero-3-phosphocholine, DMPC) yielded no acyl transfer, whereas membranes composed of lipids with unsaturated acyl chains (1,2-dioleoyl sn-glycero-3-phosphocholine, DOPC; 1-palmitoyl-2-oleoyl sn-glycero-3-phosphocholine, POPC) produced detectable lipidation activity. For all lipids, inclusion of cholesterol led to a significant increase in lipidation activity, with the greatest effect observed for 20 mol% cholesterol in POPC. In the case of membranes composed of POPC, the inclusion of cholesterol also produced small changes in the selectivity for transfer from the sn-1 vssn-2 positions of the lipid. Qualitatively, for fluid membranes, the trend in lipidation activity exhibits a positive correlation with the bending modulus of the bilayer and is accounted for in terms of the penetration depth of the peptide. Access of water to reactive intermediates also has the potential to influence lipidation rates.

Citation

Britt, H. M., Mosely, J. A., & Sanderson, J. M. (2019). The Influence of Cholesterol on Melittin Lipidation in Neutral Membranes. Physical Chemistry Chemical Physics, 21(2), 631-640. https://doi.org/10.1039/c8cp06661b

Journal Article Type Article
Acceptance Date Dec 3, 2018
Online Publication Date Dec 7, 2018
Publication Date Jan 14, 2019
Deposit Date Dec 3, 2018
Publicly Available Date Dec 3, 2019
Journal Physical Chemistry Chemical Physics
Print ISSN 1463-9076
Electronic ISSN 1463-9084
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 21
Issue 2
Pages 631-640
DOI https://doi.org/10.1039/c8cp06661b
Public URL https://durham-repository.worktribe.com/output/1312318

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