Peptide Lipidation in Lysophospholipid Micelles and Lysophospholipid-Enriched Membranes

Esmaeil, Vian S; Britt, Hannah M; Mosely, Jackie A; Sanderson, John M

Abstract

Acyl transfer from lipids to membrane-associated peptides is a well-documented process, leading to the generation of a lipidated peptide and a lysolipid. In this article, we demonstrate that acyl transfer from lysophosphatidylcholines (lysoPCs) to the peptide melittin also occurs, both in micelles of pure lysolipid and in lipid/lysolipid mixtures. In the case of bilayers containing lysolipids, acyl transfer from the lysolipid is marginally favoured over transfer from the lipid. In pure bilayers of saturated lipids, the introduction of even small amounts of lysolipid appears to significantly increase the reactivity towards lipidation.

Citation

Esmaeil, V. S., Britt, H. M., Mosely, J. A., & Sanderson, J. M. (2021). Peptide Lipidation in Lysophospholipid Micelles and Lysophospholipid-Enriched Membranes. Faraday Discussions, 2021(232), 282-294. https://doi.org/10.1039/d1fd00030f

• Journal Article Type: Article
• Acceptance Date: Jul 16, 2021
• Online Publication Date: Jul 29, 2021
• Publication Date: Dec 1, 2021
• Deposit Date: Jul 27, 2021
• Publicly Available Date: Oct 7, 2021
• Journal: Faraday Discussions
• Print ISSN: 1359-6640
• Electronic ISSN: 1364-5498
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 2021
• Issue: 232
• Pages: 282-294
• DOI: https://doi.org/10.1039/d1fd00030f
• Public URL: https://durham-repository.worktribe.com/output/1244187