17O NMR spectroscopy as a tool to study hydrogen bonding of cholesterol in lipid bilayers

Rowlands, Lucy J.; Marks, Adam; Sanderson, John M.; Law, Robert V.

Abstract

Cholesterol is a crucial component of biological membranes and can interact with other membrane components through hydrogen bonding. NMR spectroscopy has been used previously to investigate this bonding, however this study represents the first 17O NMR spectroscopy study of isotopically enriched cholesterol. We demonstrate the 17O chemical shift is dependent on hydrogen bonding, providing a novel method for the study of cholesterol in bilayers.

Citation

Rowlands, L. J., Marks, A., Sanderson, J. M., & Law, R. V. (2020). 17O NMR spectroscopy as a tool to study hydrogen bonding of cholesterol in lipid bilayers. Chemical Communications, 56(92), 14499-14502. https://doi.org/10.1039/d0cc05466f

• Journal Article Type: Article
• Acceptance Date: Oct 28, 2020
• Online Publication Date: Nov 5, 2020
• Publication Date: Nov 28, 2020
• Deposit Date: Nov 5, 2020
• Publicly Available Date: Feb 8, 2021
• Journal: Chemical Communications
• Print ISSN: 1359-7345
• Electronic ISSN: 1364-548X
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 56
• Issue: 92
• Pages: 14499-14502
• DOI: https://doi.org/10.1039/d0cc05466f