Time-resolved luminescence detection of peroxynitrite using a reactivity-based lanthanide probe

Breen, Colum; Pal, Robert; Elsegood, Mark R.J.; Teat, Simon J.; Iza, Felipe; Wende, Kristian; Buckley, Benjamin R.; Butler, Stephen J.

Abstract

Peroxynitrite (ONOO−) is a powerful and short-lived oxidant formed in vivo, which can react with most biomolecules directly. To fully understand the roles of ONOO− in cell biology, improved methods for the selective detection and real-time analysis of ONOO− are needed. We present a water-soluble, luminescent europium(III) probe for the rapid and sensitive detection of peroxynitrite in human serum, living cells and biological matrices. We have utilised the long luminescence lifetime of the probe to measure ONOO− in a time-resolved manner, effectively avoiding the influence of autofluorescence in biological samples. To demonstrate the utility of the Eu(III) probe, we monitored the production of ONOO− in different cell lines, following treatment with a cold atmospheric plasma device commonly used in the clinic for skin wound treatment.

Citation

Breen, C., Pal, R., Elsegood, M. R., Teat, S. J., Iza, F., Wende, K., Buckley, B. R., & Butler, S. J. (2020). Time-resolved luminescence detection of peroxynitrite using a reactivity-based lanthanide probe. Chemical Science, 11(12), 3164-3170. https://doi.org/10.1039/c9sc06053g

• Journal Article Type: Article
• Acceptance Date: Feb 14, 2020
• Online Publication Date: Feb 18, 2020
• Publication Date: Feb 28, 2020
• Deposit Date: Apr 15, 2020
• Publicly Available Date: Apr 15, 2020
• Journal: Chemical Science
• Print ISSN: 2041-6520
• Electronic ISSN: 2041-6539
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 11
• Issue: 12
• Pages: 3164-3170
• DOI: https://doi.org/10.1039/c9sc06053g
• Public URL: https://durham-repository.worktribe.com/output/1273318