Skip to main content

Research Repository

Advanced Search

CYK4 relaxes the bias in the off-axis motion by MKLP1 kinesin-6

Maruyama, Yohei; Sugawa, Mitsuhiro; Yamaguchi, Shin; Davies, Tim; Osaki, Toshihisa; Kobayashi, Takuya; Yamagishi, Masahiko; Takeuchi, Shoji; Mishima, Masanori; Yajima, Junichiro

CYK4 relaxes the bias in the off-axis motion by MKLP1 kinesin-6 Thumbnail


Authors

Yohei Maruyama

Mitsuhiro Sugawa

Shin Yamaguchi

Toshihisa Osaki

Takuya Kobayashi

Masahiko Yamagishi

Shoji Takeuchi

Masanori Mishima

Junichiro Yajima



Abstract

Centralspindlin, a complex of the MKLP1 kinesin-6 and CYK4 GAP subunits, plays key roles in metazoan cytokinesis. CYK4-binding to the long neck region of MKLP1 restricts the configuration of the two MKLP1 motor domains in the centralspindlin. However, it is unclear how the CYK4-binding modulates the interaction of MKLP1 with a microtubule. Here, we performed three-dimensional nanometry of a microbead coated with multiple MKLP1 molecules on a freely suspended microtubule. We found that beads driven by dimeric MKLP1 exhibited persistently left-handed helical trajectories around the microtubule axis, indicating torque generation. By contrast, centralspindlin, like monomeric MKLP1, showed similarly left-handed but less persistent helical movement with occasional rightward movements. Analysis of the fluctuating helical movement indicated that the MKLP1 stochastically makes off-axis motions biased towards the protofilament on the left. CYK4-binding to the neck domains in MKLP1 enables more flexible off-axis motion of centralspindlin, which would help to avoid obstacles along crowded spindle microtubules.

Citation

Maruyama, Y., Sugawa, M., Yamaguchi, S., Davies, T., Osaki, T., Kobayashi, T., …Yajima, J. (2021). CYK4 relaxes the bias in the off-axis motion by MKLP1 kinesin-6. Communications Biology, 4, Article 180. https://doi.org/10.1038/s42003-021-01704-2

Journal Article Type Article
Acceptance Date Jan 14, 2021
Online Publication Date Feb 10, 2021
Publication Date 2021
Deposit Date Mar 11, 2021
Publicly Available Date Jun 14, 2021
Journal Communications Biology
Publisher Nature Research
Peer Reviewed Peer Reviewed
Volume 4
Article Number 180
DOI https://doi.org/10.1038/s42003-021-01704-2
Public URL https://durham-repository.worktribe.com/output/1251339

Files

Published Journal Article (2.9 Mb)
PDF

Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/

Copyright Statement
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.






You might also like



Downloadable Citations