Targeted pH switched europium complexes monitoring receptor internalisation in living cells

Fradgley, Jack D.; Starck, Matthieu; Laget, Michel; Bourrier, Emmanuel; Dupuis, Elodie; Lamarque, Laurent; Trinquet, Eric; Zwier, Jurriaan M.; Parker, David

Abstract

We report the design and evaluation of pH responsive luminescent europium(III) probes that allow conjugation to targeting vectors to monitor receptor internalisation in cells. The approach adopted here can be used to tag proteins selectively and to monitor uptake into more acidic organelles, thereby enhancing the performance of time-resolved internalisation assays that require pH monitoring in real time.

Citation

Fradgley, J. D., Starck, M., Laget, M., Bourrier, E., Dupuis, E., Lamarque, L., Trinquet, E., Zwier, J. M., & Parker, D. (2021). Targeted pH switched europium complexes monitoring receptor internalisation in living cells. Chemical Communications, 57(47), 5814-5817. https://doi.org/10.1039/d1cc01029h

• Journal Article Type: Article
• Acceptance Date: May 4, 2021
• Online Publication Date: May 4, 2021
• Publication Date: 2021
• Deposit Date: Aug 25, 2021
• Publicly Available Date: Aug 25, 2021
• Journal: Chemical Communications
• Print ISSN: 1359-7345
• Electronic ISSN: 1364-548X
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 57
• Issue: 47
• Pages: 5814-5817
• DOI: https://doi.org/10.1039/d1cc01029h
• Public URL: https://durham-repository.worktribe.com/output/1243100