Paige E Panter
Cell-wall fucosylation in Arabidopsis influences control of leaf water loss and alters stomatal development and mechanical properties
Panter, Paige E; Seifert, Jacob; Dale, Maeve; Pridgeon, Ashley J; Hulme, Rachel; Ramsay, Nathan; Contera, Sonia; Knight, Heather
Authors
Jacob Seifert
Maeve Dale
Ashley J Pridgeon
Rachel Hulme
Nathan Ramsay nathan.ramsay@durham.ac.uk
PGR Student Doctor of Philosophy
Sonia Contera
Professor Heather Knight p.h.knight@durham.ac.uk
Professor
Abstract
The Arabidopsis sensitive-to-freezing8 (sfr8) mutant exhibits reduced cell-wall (CW) fucose levels and compromised freezing tolerance. To examine whether CW fucosylation affects the response to desiccation also, we tested the effect of leaf excision in sfr8 and the allelic mutant mur1-1. Leaf water loss was strikingly higher than wild type in these, but not other, fucosylation mutants. We hypothesised that reduced fucosylation in guard cell (GC) walls might limit stomatal closure through altering mechanical properties. Multifrequency atomic force microscopy (AFM) measurements revealed a reduced elastic modulus (E’), representing reduced stiffness, in sfr8 GC walls. Interestingly, however, we discovered a compensatory mechanism whereby a concomitant reduction in the storage modulus (E’’) maintained a wild type viscoelastic time response (tau) in sfr8. Stomata in intact leaf discs of sfr8 responded normally to a closure stimulus, ABA, suggesting the time response may relate more to closure properties than stiffness does. sfr8 stomatal pore complexes were larger than wild type and GCs lacked a fully developed cuticular ledge, both potential contributors to the greater leaf water loss in sfr8. We present data that indicate fucosylation-dependent dimerisation of the CW pectic domain rhamnogalacturonan-II may be essential for normal cuticular ledge development and leaf water retention.
Citation
Panter, P. E., Seifert, J., Dale, M., Pridgeon, A. J., Hulme, R., Ramsay, N., Contera, S., & Knight, H. (2023). Cell-wall fucosylation in Arabidopsis influences control of leaf water loss and alters stomatal development and mechanical properties. Journal of Experimental Botany, 74(8), 2680-2691. https://doi.org/10.1093/jxb/erad039
Journal Article Type | Article |
---|---|
Online Publication Date | Feb 21, 2023 |
Publication Date | Apr 18, 2023 |
Deposit Date | Feb 3, 2023 |
Publicly Available Date | May 30, 2023 |
Journal | Journal of Experimental Botany |
Print ISSN | 0022-0957 |
Electronic ISSN | 1460-2431 |
Publisher | Oxford University Press |
Peer Reviewed | Peer Reviewed |
Volume | 74 |
Issue | 8 |
Pages | 2680-2691 |
DOI | https://doi.org/10.1093/jxb/erad039 |
Public URL | https://durham-repository.worktribe.com/output/1183813 |
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Copyright Statement
© The Author(s) 2023. Published by Oxford University Press on behalf of the Society for Experimental Biology.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
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Publisher Licence URL
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