Understanding pectin cross-linking in plant cell walls

Obomighie, Irabonosi; Prentice, Iain J.; Lewin-Jones, Peter; Bachtiger, Fabienne; Ramsay, Nathan; Kishi-Itakura, Chieko; Goldberg, Martin W.; Hawkins, Tim J.; Sprittles, James E.; Knight, Heather; Sosso, Gabriele C.

Abstract

Pectin is a major component of plant cells walls. The extent to which pectin chains crosslink with one another determines crucial properties including cell wall strength, porosity, and the ability of small, biologically significant molecules to access the cell. Despite its importance, significant gaps remain in our comprehension, at the molecular level, of how pectin cross-links influence the mechanical and physical properties of cell walls. This study employs a multidisciplinary approach, combining molecular dynamics simulations, experimental investigations, and mathematical modelling, to elucidate the mechanism of pectin cross-linking and its effect on cell wall porosity. The computational aspects of this work challenge the prevailing egg-box model, favoring instead a zipper model for pectin cross-linking, whilst our experimental work highlights the significant impact of pectin cross-linking on cell wall porosity. This work advances our fundamental understanding of the biochemistry underpinning the structure and function of the plant cell wall. This knowledge has important implications for agricultural biotechnology, informing us about the chemical properties of plant pectins that are best suited for improving crop resilience and amenability to biofuel extraction by modifying the cell wall.

Citation

Obomighie, I., Prentice, I. J., Lewin-Jones, P., Bachtiger, F., Ramsay, N., Kishi-Itakura, C., Goldberg, M. W., Hawkins, T. J., Sprittles, J. E., Knight, H., & Sosso, G. C. (2025). Understanding pectin cross-linking in plant cell walls. Communications Biology, 8(1), Article 72. https://doi.org/10.1038/s42003-025-07495-0

• Journal Article Type: Article
• Acceptance Date: Jan 8, 2025
• Online Publication Date: Jan 17, 2025
• Publication Date: Jan 17, 2025
• Deposit Date: Jan 20, 2025
• Publicly Available Date: Jan 20, 2025
• Journal: Communications Biology
• Electronic ISSN: 2399-3642
• Publisher: Nature Research
• Peer Reviewed: Peer Reviewed
• Volume: 8
• Issue: 1
• Article Number: 72
• DOI: https://doi.org/10.1038/s42003-025-07495-0
• Public URL: https://durham-repository.worktribe.com/output/3341847