Adaptive optical methods for in vivo imaging in developing Zebra fish

Girkin, JM; Taylor, JM; Bourgenot, C; Saunter, CD; Love, GD

Abstract

The humble Zebra fish is rapidly establishing itself as the model of choice for a wide range of biological investigations, in particular at the developing embryo stage. Single Plane Illumination Microscopy (SPIM) has already been shown to be a very powerful method with which to image such samples over extended periods of time. However, the sample is only around 2mm long and its physical structure heavily influenced by the beating heart with the circulation of blood causing movement throughout the fish on the scale of several microns. The paper reports on the development of an active synchronization method, which optically “freezes” motion and significantly reduces light induced toxicity and bleaching within the sample. This has been combined with adaptive optics to remove both instrument and sample induced aberrations enabling live high resolution imaging within the beating heart with micron resolution over extended time periods.

Citation

Girkin, J., Taylor, J., Bourgenot, C., Saunter, C., & Love, G. (2012). Adaptive optical methods for in vivo imaging in developing Zebra fish. In 2012 International Symposium on Optomechatronic Technologies (ISOT 2012). https://doi.org/10.1109/isot.2012.6403295

• Conference Name: 2012 International Symposium on Optomechatronic Technologies (ISOT 2012)
• Conference Location: Paris
• Online Publication Date: Jan 7, 2013
• Publication Date: 2012
• Deposit Date: Apr 22, 2022
• Publisher: Institute of Electrical and Electronics Engineers
• Book Title: 2012 International Symposium on Optomechatronic Technologies (ISOT 2012)
• DOI: https://doi.org/10.1109/isot.2012.6403295