Outputs (8)

Two Distinct Thermodynamic Gradients for Cellular Metalation of Vitamin B12 (2023)
Journal Article
Young, T. R., Deery, E., Foster, A. W., Martini, M. A., Osman, D., Warren, M. J., & Robinson, N. J. (2023). Two Distinct Thermodynamic Gradients for Cellular Metalation of Vitamin B12. JACS Au, 3(5), 1264-1534. https://doi.org/10.1021/jacsau.3c00119

The acquisition of CoII by the corrin component of vitamin B12 follows one of two distinct pathways, referred to as early or late CoII insertion. The late insertion pathway exploits a CoII metallochaperone (CobW) from the COG0523 family of G3E GTPase... Read More about Two Distinct Thermodynamic Gradients for Cellular Metalation of Vitamin B12.

Protein metalation in a nutshell (2022)
Journal Article
Osman, D., & Robinson, N. J. (2023). Protein metalation in a nutshell. FEBS Letters, 597(1), 141-150. https://doi.org/10.1002/1873-3468.14500

Metalation, the acquisition of metals by proteins, must avoid mis-metalation with tighter binding metals. This is illustrated by four selected proteins that require different metals: all show similar ranked orders of affinity for bioavailable metals,... Read More about Protein metalation in a nutshell.

Insights into the antibacterial mechanism of action of chelating agents by selective deprivation of iron, manganese and zinc (2021)
Journal Article
Paterson, J. R., Beecroft, M. S., Mulla, R. S., Osman, D., Reeder, N. L., Caserta, J. A., Young, T. R., Pettigrew, C. A., Davies, G. E., Williams, J. G., & Sharples, G. J. (2022). Insights into the antibacterial mechanism of action of chelating agents by selective deprivation of iron, manganese and zinc. Applied and Environmental Microbiology, 88(2), Article e01641-21. https://doi.org/10.1128/aem.01641-21

Bacterial growth and proliferation can be restricted by limiting the availability of metal ions in their environment. Humans sequester iron, manganese and zinc to help prevent infection by pathogens, a system termed nutritional immunity. Commercially... Read More about Insights into the antibacterial mechanism of action of chelating agents by selective deprivation of iron, manganese and zinc.

Bacterial sensors define intracellular free energies for correct enzyme metalation (2019)
Journal Article
Osman, D., Martini, M. A., Foster, A. W., Chen, J., Scott, A. J., Morton, R. J., Steed, J. W., Lurie-Luke, E., Huggins, T. G., Lawrence, A. D., Deery, E., Warren, M. J., Chivers, P. T., & Robinson, N. J. (2019). Bacterial sensors define intracellular free energies for correct enzyme metalation. Nature Chemical Biology, 15(3), 241-249. https://doi.org/10.1038/s41589-018-0211-4

There is a challenge for metalloenzymes to acquire their correct metals because some inorganic elements form more stable complexes with proteins than do others. These preferences can be overcome provided some metals are more available than others. Ho... Read More about Bacterial sensors define intracellular free energies for correct enzyme metalation.

A unique ferredoxin acts as a player in the low-iron response of photosynthetic organisms (2018)
Journal Article
Schorsch, M., Kramer, M., Goss, T., Eisenhut, M., Robinson, N., Osman, D., Wilde, A., Sadaf, S., Brückler, H., Walder, L., Scheibe, R., Hase, T., & Hanke, G. T. (2018). A unique ferredoxin acts as a player in the low-iron response of photosynthetic organisms. Proceedings of the National Academy of Sciences, 115(51), E12111-E12120. https://doi.org/10.1073/pnas.1810379115

Iron chronically limits aquatic photosynthesis, especially in marine environments, and the correct perception and maintenance of iron homeostasis in photosynthetic bacteria, including cyanobacteria, is therefore of global significance. Multiple adapt... Read More about A unique ferredoxin acts as a player in the low-iron response of photosynthetic organisms.

Fine control of metal concentrations is necessary for cells to discern zinc from cobalt (2017)
Journal Article
Osman, D., Foster, A. W., Chen, J., Svedaite, K., Steed, J. W., Lurie-Luke, E., …Robinson, N. J. (2017). Fine control of metal concentrations is necessary for cells to discern zinc from cobalt. Nature Communications, 8, Article 1884. https://doi.org/10.1038/s41467-017-02085-z

Bacteria possess transcription factors whose DNA-binding activity is altered upon binding to specific metals, but metal binding is not specific in vitro. Here we show that tight regulation of buffered intracellular metal concentrations is a prerequis... Read More about Fine control of metal concentrations is necessary for cells to discern zinc from cobalt.

The Effectors and Sensory Sites of Formaldehyde-Responsive Regulator FrmR and Metal-Sensing Variant (2016)
Journal Article
Osman, D., Piergentili, C., Chen, J., Sayer, L., Usón, I., Huggins, T., …Pohl, E. (2016). The Effectors and Sensory Sites of Formaldehyde-Responsive Regulator FrmR and Metal-Sensing Variant. Journal of Biological Chemistry, 291(37), 19502-19516. https://doi.org/10.1074/jbc.m116.745174

The DUF156 family of DNA-binding, transcriptional-regulators include metal-sensors which respond to cobalt and/or nickel (RcnR, InrS) or copper (CsoR), plus CstR which responds to persulfide, and formaldehyde-responsive FrmR. Unexpectedly, the allost... Read More about The Effectors and Sensory Sites of Formaldehyde-Responsive Regulator FrmR and Metal-Sensing Variant.

Metal Preferences and Metallation (2014)
Journal Article
Foster, A. W., Osman, D., & Robinson, N. J. (2014). Metal Preferences and Metallation. Journal of Biological Chemistry, 289(41), 28095-28103. https://doi.org/10.1074/jbc.r114.588145

The metal-binding preferences of most metalloproteins do not match their metal-requirements. Thus, metallation of an estimated 30% of metalloenzymes is aided by metal-delivery systems, with ~25% acquiring pre-assembled metal-cofactors. The remaining... Read More about Metal Preferences and Metallation.