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Fine control of metal concentrations is necessary for cells to discern zinc from cobalt

Osman, Deenah; Foster, Andrew W.; Chen, Junjun; Svedaite, Kotryna; Steed, Jonathan W.; Lurie-Luke, Elena; Huggins, Thomas G.; Robinson, Nigel J.

Fine control of metal concentrations is necessary for cells to discern zinc from cobalt Thumbnail


Authors

Deenah Osman

Andrew W. Foster

Junjun Chen

Kotryna Svedaite

Elena Lurie-Luke

Thomas G. Huggins



Abstract

Bacteria possess transcription factors whose DNA-binding activity is altered upon binding to specific metals, but metal binding is not specific in vitro. Here we show that tight regulation of buffered intracellular metal concentrations is a prerequisite for metal specificity of Zur, ZntR, RcnR and FrmR in Salmonella Typhimurium. In cells, at non-inhibitory elevated concentrations, Zur and ZntR, only respond to Zn(II), RcnR to cobalt and FrmR to formaldehyde. However, in vitro all these sensors bind non-cognate metals, which alters DNA binding. We model the responses of these sensors to intracellular-buffered concentrations of Co(II) and Zn(II) based upon determined abundances, metal affinities and DNA affinities of each apo- and metalated sensor. The cognate sensors are modelled to respond at the lowest concentrations of their cognate metal, explaining specificity. However, other sensors are modelled to respond at concentrations only slightly higher, and cobalt or Zn(II) shock triggers mal-responses that match these predictions. Thus, perfect metal specificity is fine-tuned to a narrow range of buffered intracellular metal concentrations.

Journal Article Type Article
Acceptance Date Nov 6, 2017
Online Publication Date Dec 1, 2017
Publication Date Dec 1, 2017
Deposit Date Oct 27, 2017
Publicly Available Date Dec 1, 2017
Journal Nature Communications
Publisher Nature Research
Peer Reviewed Peer Reviewed
Volume 8
Article Number 1884
DOI https://doi.org/10.1038/s41467-017-02085-z
Public URL https://durham-repository.worktribe.com/output/1341658

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http://creativecommons.org/licenses/by/4.0/

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This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commonslicense, unless indicated otherwise in a credit line to the material. If material is not included in the article’sCreative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.






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