Michael Schorsch
A unique ferredoxin acts as a player in the low-iron response of photosynthetic organisms
Schorsch, Michael; Kramer, Manuela; Goss, Tatjana; Eisenhut, Marion; Robinson, Nigel; Osman, Deenah; Wilde, Annegret; Sadaf, Shamaila; Brückler, Hendrik; Walder, Lorenz; Scheibe, Renate; Hase, Toshiharu; Hanke, Guy T.
Authors
Manuela Kramer
Tatjana Goss
Marion Eisenhut
Professor Nigel Robinson nigel.robinson@durham.ac.uk
Emeritus Professor
Deenah Osman
Annegret Wilde
Shamaila Sadaf
Hendrik Brückler
Lorenz Walder
Renate Scheibe
Toshiharu Hase
Guy T. Hanke
Abstract
Iron chronically limits aquatic photosynthesis, especially in marine environments, and the correct perception and maintenance of iron homeostasis in photosynthetic bacteria, including cyanobacteria, is therefore of global significance. Multiple adaptive mechanisms, responsive promoters, and posttranscriptional regulators have been identified, which allow cyanobacteria to respond to changing iron concentrations. However, many factors remain unclear, in particular, how iron status is perceived within the cell. Here we describe a cyanobacterial ferredoxin (Fed2), with a unique C-terminal extension, that acts as a player in iron perception. Fed2 homologs are highly conserved in photosynthetic organisms from cyanobacteria to higher plants, and, although they belong to the plant type ferredoxin family of [2Fe-2S] photosynthetic electron carriers, they are not involved in photosynthetic electron transport. As deletion of fed2 appears lethal, we developed a C-terminal truncation system to attenuate protein function. Disturbed Fed2 function resulted in decreased chlorophyll accumulation, and this was exaggerated in iron-depleted medium, where different truncations led to either exaggerated or weaker responses to low iron. Despite this, iron concentrations remained the same, or were elevated in all truncation mutants. Further analysis established that, when Fed2 function was perturbed, the classical iron limitation marker IsiA failed to accumulate at transcript and protein levels. By contrast, abundance of IsiB, which shares an operon with isiA, was unaffected by loss of Fed2 function, pinpointing the site of Fed2 action in iron perception to the level of posttranscriptional regulation.
Citation
Schorsch, M., Kramer, M., Goss, T., Eisenhut, M., Robinson, N., Osman, D., …Hanke, G. T. (2018). A unique ferredoxin acts as a player in the low-iron response of photosynthetic organisms. Proceedings of the National Academy of Sciences, 115(51), E12111-E12120. https://doi.org/10.1073/pnas.1810379115
Journal Article Type | Article |
---|---|
Acceptance Date | Nov 5, 2018 |
Online Publication Date | Dec 4, 2018 |
Publication Date | Dec 4, 2018 |
Deposit Date | Nov 6, 2018 |
Publicly Available Date | Jan 4, 2019 |
Journal | Proceedings of the National Academy of Sciences |
Print ISSN | 0027-8424 |
Electronic ISSN | 1091-6490 |
Publisher | National Academy of Sciences |
Peer Reviewed | Peer Reviewed |
Volume | 115 |
Issue | 51 |
Pages | E12111-E12120 |
DOI | https://doi.org/10.1073/pnas.1810379115 |
Public URL | https://durham-repository.worktribe.com/output/1309669 |
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Copyright Statement
This open access article is distributed under Creative Commons Attribution-NonCommercialNoDerivatives License 4.0 (CC BY-NC-ND).
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