Processing of intermediates in recombination and DNA repair: identification of a new endonuclease that specifically cleaves Holliday junctions. (1994)
Journal Article
Sharples, G., Chan, S., Mahdi, A., Whitby, M., & Lloyd, R. (1994). Processing of intermediates in recombination and DNA repair: identification of a new endonuclease that specifically cleaves Holliday junctions. The EMBO Journal, 13(24), 6133-6142

The formation and subsequent resolution of Holliday junctions are critical stages in recombination. We describe a new Escherichia coli endonuclease that resolves Holliday intermediates by junction cleavage. The 14 kDa Rus protein binds DNA containing... Read More about Processing of intermediates in recombination and DNA repair: identification of a new endonuclease that specifically cleaves Holliday junctions..

A mutation in helicase motif III of E. coli RecG protein abolishes branch migration of Holliday junctions. (1994)
Journal Article
Sharples, G., Whitby, M., Ryder, L., & Lloyd, R. (1994). A mutation in helicase motif III of E. coli RecG protein abolishes branch migration of Holliday junctions. Nucleic Acids Research, 22(3), 308-313

The RecG protein of Escherichia coli catalyses branch migration of Holliday junctions made by RecA and dissociates synthetic X junctions into duplex products in reactions that require hydrolysis of ATP. To investigate the mode of action of this enzym... Read More about A mutation in helicase motif III of E. coli RecG protein abolishes branch migration of Holliday junctions..

Cloning, overexpression, purification, and characterization of the Escherichia coli RuvC Holliday junction resolvase. (1994)
Journal Article
Dunderdale, H., Sharples, G., Lloyd, R., & West, S. (1994). Cloning, overexpression, purification, and characterization of the Escherichia coli RuvC Holliday junction resolvase. Journal of Biological Chemistry, 269(7), 5187-5194

The ruvC gene has been cloned into the plasmid pT7-7 under the control of the T7 phi 10 promoter. Following induction with isopropyl-1-thio-beta-D-galactopyranoside, the 19-kDa RuvC protein was overexpressed to 20-30% of total cell protein. RuvC has... Read More about Cloning, overexpression, purification, and characterization of the Escherichia coli RuvC Holliday junction resolvase..