Mutagenesis of squash (Cucurbita moschata) glycerol-3-phosphate acyltransferase (GPAT) to produce an enzyme with altered substrate selectivity
(2000)
Presentation / Conference Contribution
Hayman, M., Fawcett, T., Schierer, T., Simon, J., Kroon, J., Gilroy, J., Rice, D., Rafferty, J., Turnbull, A., Sedelnikova, S., & Slabas, A. Mutagenesis of squash (Cucurbita moschata) glycerol-3-phosphate acyltransferase (GPAT) to produce an enzyme with altered substrate selectivity
In an attempt to rationalize the relationship between structure and substrate selectivity of glycerol-3-phosphate acyltransferase (GPAT, 1AT, EC 2.3.1.15) we have cloned a number of cDNAs into the PET overexpression system using a PCR-based approach.... Read More about Mutagenesis of squash (Cucurbita moschata) glycerol-3-phosphate acyltransferase (GPAT) to produce an enzyme with altered substrate selectivity.