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Dr Johannes Kroon's Outputs (38)

NET4 and RabG3 link actin to the tonoplast and facilitate cytoskeletal remodelling during stomatal immunity (2023)
Journal Article
Hawkins, T. J., Kopischke, M., Duckney, P. J., Rybak, K., Mentlak, D. A., Kroon, J. T. M., …Robatzek, S. (2023). NET4 and RabG3 link actin to the tonoplast and facilitate cytoskeletal remodelling during stomatal immunity. Nature Communications, 14(1), Article 5848. https://doi.org/10.1038/s41467-023-41337-z

Members of the NETWORKED (NET) family are involved in actin-membrane interactions. Here we show that two members of the NET family, NET4A and NET4B, are essential for normal guard cell actin reorganization, which is a process critical for stomatal cl... Read More about NET4 and RabG3 link actin to the tonoplast and facilitate cytoskeletal remodelling during stomatal immunity.

Exo84c interacts with VAP27 to regulate exocytotic compartment degradation and stigma senescence (2023)
Journal Article
Zhang, T., Li, Y., Li, C., Zang, J., Gao, E., Kroon, J. T., …Wang, P. (2023). Exo84c interacts with VAP27 to regulate exocytotic compartment degradation and stigma senescence. Nature Communications, 14(1), Article 4888. https://doi.org/10.1038/s41467-023-40729-5

In plants, exocyst subunit isoforms exhibit significant functional diversity in that they are involved in either protein secretion or autophagy, both of which are essential for plant development and survival. Although the molecular basis of autophagy... Read More about Exo84c interacts with VAP27 to regulate exocytotic compartment degradation and stigma senescence.

TraB family proteins are components of ER-mitochondrial contact sites and regulate ER-mitochondrial interactions and mitophagy (2022)
Journal Article
Li, C., Duckney, P., Zhang, T., Fu, Y., Li, X., Kroon, J., De Jaeger, G., Cheng, Y., Hussey, P. J., & Wang, P. (2022). TraB family proteins are components of ER-mitochondrial contact sites and regulate ER-mitochondrial interactions and mitophagy. Nature Communications, 13, Article 5658. https://doi.org/10.1038/s41467-022-33402-w

ER-mitochondria contact sites (EMCSs) are important for mitochondrial function. Here, we have identified a EMCS complex, comprising a family of uncharacterised mitochondrial outer membrane proteins, TRB1, TRB2 and the ER protein, VAP27-1. In Arabidop... Read More about TraB family proteins are components of ER-mitochondrial contact sites and regulate ER-mitochondrial interactions and mitophagy.

Extracellular ATP targets Arabidopsis RIBONUCLEASE 1 to suppress mycotoxin stress-induced cell death (2022)
Journal Article
Goodman, H. L., Kroon, J. T., Tomé, D. F., Hamilton, J. M., Alqarni, A. O., & Chivasa, S. (2022). Extracellular ATP targets Arabidopsis RIBONUCLEASE 1 to suppress mycotoxin stress-induced cell death. New Phytologist, 235(4), 1531-1542. https://doi.org/10.1111/nph.18211

Extracellular ATP is a purinergic signal with important functions in regulating plant growth and stress-adaptive responses, including programmed cell death. While signalling events proximate to receptor activation at the plasma membrane have been cha... Read More about Extracellular ATP targets Arabidopsis RIBONUCLEASE 1 to suppress mycotoxin stress-induced cell death.

NETWORKED2‐Subfamily Proteins Regulate the Cortical Actin Cytoskeleton of Growing Pollen Tubes and Polarised Pollen Tube Growth (2021)
Journal Article
Duckney, P., Kroon, J. T., Dixon, M. R., Hawkins, T. J., Deeks, M. J., & Hussey, P. J. (2021). NETWORKED2‐Subfamily Proteins Regulate the Cortical Actin Cytoskeleton of Growing Pollen Tubes and Polarised Pollen Tube Growth. New Phytologist, 231(1), 152-164. https://doi.org/10.1111/nph.17391

We have recently characterised NET2A as a pollen‐specific actin‐binding protein which binds F‐actin at the plasma membrane of growing pollen tubes. However, the role of NET2 proteins in pollen development and fertilisation have yet to be elucidated.... Read More about NETWORKED2‐Subfamily Proteins Regulate the Cortical Actin Cytoskeleton of Growing Pollen Tubes and Polarised Pollen Tube Growth.

Isolation of Arabidopsis extracellular ATP‐binding proteins by affinity proteomics and identification of PHOSPHOLIPASE C‐LIKE 1 as an extracellular protein essential for fumonisin B1 toxicity (2021)
Journal Article
Smith, S. J., Goodman, H., Kroon, J. T., Brown, A. P., Simon, W. J., & Chivasa, S. (2021). Isolation of Arabidopsis extracellular ATP‐binding proteins by affinity proteomics and identification of PHOSPHOLIPASE C‐LIKE 1 as an extracellular protein essential for fumonisin B1 toxicity. The Plant Journal, 106(5), 1387-1400. https://doi.org/10.1111/tpj.15243

ATP is secreted to the extracellular matrix where it activates plasma membrane receptors for controlling plant growth and stress‐adaptive processes. DOES NOT RESPOND TO NUCLEOTIDES 1 (DORN1), the first plant ATP receptor was identified, but key downs... Read More about Isolation of Arabidopsis extracellular ATP‐binding proteins by affinity proteomics and identification of PHOSPHOLIPASE C‐LIKE 1 as an extracellular protein essential for fumonisin B1 toxicity.

A PXY-Mediated Transcriptional Network Integrates Signaling Mechanisms to Control Vascular Development in Arabidopsis (2019)
Journal Article
Smit, M., McGregor, S., Sun, H., Gough, C., Bågman, A.-M., Soyars, C., Kroon, J., Gaudinier, A., Williams, C., Yang, X., Nimchuk, Z., Weijers, D., Turner, S., Brady, S., & Etchells, J. (2020). A PXY-Mediated Transcriptional Network Integrates Signaling Mechanisms to Control Vascular Development in Arabidopsis. The Plant Cell, 32, 319-335. https://doi.org/10.1105/tpc.19.00562

Vascular meristems generate the majority of biomass in higher plants. They constitute a bifacial stem cell population from which xylem and phloem are specified on opposing sides by positional signals. The PHLOEM INTERCALATED WITH XYLEM (PXY) receptor... Read More about A PXY-Mediated Transcriptional Network Integrates Signaling Mechanisms to Control Vascular Development in Arabidopsis.

Organ-specific genetic interactions between paralogues of the PXY and ER receptor kinases enforce radial patterning in Arabidopsis vascular tissue (2019)
Journal Article
Wang, N., Bagdassarian, K., Doherty, R., Kroon, J., Connor, K., Wang, X., Wang, W., Jermyn, I., Turner, S., & Etchells, J. (2019). Organ-specific genetic interactions between paralogues of the PXY and ER receptor kinases enforce radial patterning in Arabidopsis vascular tissue. Development, 146(10), Article 177105. https://doi.org/10.1242/dev.177105

In plants, cells do not migrate. Tissues are frequently arranged in concentric rings, thus expansion of inner layers is coordinated with cell division and/or expansion of cells in outer layers. In Arabidopsis stems, receptor kinases, PXY and ER, gene... Read More about Organ-specific genetic interactions between paralogues of the PXY and ER receptor kinases enforce radial patterning in Arabidopsis vascular tissue.

Paralogues of the PXY and ER receptor kinases enforce radial patterning in plant vascular tissue (2018)
Journal Article
Wang, N., Bagdassarian, K. S., Doherty, R. E., Wang, X. Y., Kroon, J. T., Wang, W., Jermyn, I. H., Turner, S. R., & Etchells, J. P. Paralogues of the PXY and ER receptor kinases enforce radial patterning in plant vascular tissue. https://doi.org/10.1101/357244. Manuscript submitted for publication

Plant cell walls do not allow cells to migrate, thus plant growth and development is entirely the consequence of changes to cell division and cell elongation. Where tissues are arranged in concentric rings, expansion of inner tissue, such as that whi... Read More about Paralogues of the PXY and ER receptor kinases enforce radial patterning in plant vascular tissue.

Actin–membrane interactions mediated by NETWORKED2 in Arabidopsis pollen tubes through associations with Pollen Receptor-Like Kinase 4 and 5 (2017)
Journal Article
Duckney, P., Deeks, M., Dixon, M., Kroon, J., Hawkins, T., & Hussey, P. (2017). Actin–membrane interactions mediated by NETWORKED2 in Arabidopsis pollen tubes through associations with Pollen Receptor-Like Kinase 4 and 5. New Phytologist, 216(4), 1170-1180. https://doi.org/10.1111/nph.14745

During fertilization, Pollen Receptor-Like Kinases (PRKs) control pollen tube growth through the pistil in response to extracellular signals, and regulate the actin cytoskeleton at the tube apex to drive tip growth. We investigated a novel link betwe... Read More about Actin–membrane interactions mediated by NETWORKED2 in Arabidopsis pollen tubes through associations with Pollen Receptor-Like Kinase 4 and 5.

Cyclin-dependent kinase activity enhances phosphatidylcholine biosynthesis in Arabidopsis by repressing phosphatidic acid phosphohydrolase activity (2016)
Journal Article
Craddock, C. P., Adams, N., Kroon, J. T., Bryant, F. M., Hussey, P. J., Kurup, S., & Eastmond, P. J. (2017). Cyclin-dependent kinase activity enhances phosphatidylcholine biosynthesis in Arabidopsis by repressing phosphatidic acid phosphohydrolase activity. The Plant Journal, 89(1), 3-14. https://doi.org/10.1111/tpj.13321

Coordination of endomembrane biogenesis with cell cycle progression is considered to be important in maintaining cell function during growth and development. We previously showed that disruption of PHOSPHATIDIC ACID PHOSPHOHYDROLASE (PAH) activity in... Read More about Cyclin-dependent kinase activity enhances phosphatidylcholine biosynthesis in Arabidopsis by repressing phosphatidic acid phosphohydrolase activity.

A Novel Function for Arabidopsis CYCLASE1 in Programmed Cell Death Revealed by Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) Analysis of Extracellular Matrix Proteins (2015)
Journal Article
Smith, S. J., Kroon, J. T., Simon, W. J., Slabas, A. R., & Chivasa, S. (2015). A Novel Function for Arabidopsis CYCLASE1 in Programmed Cell Death Revealed by Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) Analysis of Extracellular Matrix Proteins. Molecular and Cellular Proteomics, 14(6), 1556-1568. https://doi.org/10.1074/mcp.m114.045054

Programmed cell death is essential for plant development and stress adaptation. A detailed understanding of the signal transduction pathways that regulate plant programmed cell death requires identification of the underpinning protein networks. Here,... Read More about A Novel Function for Arabidopsis CYCLASE1 in Programmed Cell Death Revealed by Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) Analysis of Extracellular Matrix Proteins.

Tissue-specific whole transcriptome sequencing in castor, directed at understanding triacylglycerol lipid biosynthetic pathways (2012)
Journal Article
Brown, A., Kroon, J., Swarbreck, D., Febrer, M., Larson, T., Graham, I., …Slabas, A. (2012). Tissue-specific whole transcriptome sequencing in castor, directed at understanding triacylglycerol lipid biosynthetic pathways. PLoS ONE, 7(2), Article e30100. https://doi.org/10.1371/journal.pone.0030100

Background: Storage triacylglycerols in castor bean seeds are enriched in the hydroxylated fatty acid ricinoleate. Extensive tissue-specific RNA-Seq transcriptome and lipid analysis will help identify components important for its biosynthesis. Method... Read More about Tissue-specific whole transcriptome sequencing in castor, directed at understanding triacylglycerol lipid biosynthetic pathways.

Components of Complex Lipid Biosynthetic Pathways in Developing Castor (Ricinus communis) Seeds Identified by MudPIT Analysis of Enriched Endoplasmic Reticulum (2011)
Journal Article
Brown, A., Kroon, J., Topping, J., Robson, J., Simon, W., & Slabas, A. (2011). Components of Complex Lipid Biosynthetic Pathways in Developing Castor (Ricinus communis) Seeds Identified by MudPIT Analysis of Enriched Endoplasmic Reticulum. Journal of Proteome Research, 10(8), 3565-3577. https://doi.org/10.1021/pr2002066

Recessive mutations in the putative calcium-activated chloride channel Anoctamin 5 cause proximal LGMD2L and distal MMD3 muscular dystrophies. (2010)
Journal Article
Bolduc, V., Marlow, G., Boycott, K., Saleki, K., Inoue, H., Kroon, J., …Brais, B. (2010). Recessive mutations in the putative calcium-activated chloride channel Anoctamin 5 cause proximal LGMD2L and distal MMD3 muscular dystrophies. American Journal of Human Genetics, 86(2), 213-221. https://doi.org/10.1016/j.ajhg.2009.12.013

The recently described human anion channel Anoctamin (ANO) protein family comprises at least ten members, many of which have been shown to correspond to calcium-activated chloride channels. To date, the only reported human mutations in this family of... Read More about Recessive mutations in the putative calcium-activated chloride channel Anoctamin 5 cause proximal LGMD2L and distal MMD3 muscular dystrophies..

Identification and functional expression of a type 2 acyl-CoA :diacylglycerol acyltransferase (DGAT2) in developing castor bean seedswhich has high homology to the major triglyceride biosynthetic enzymeof fungi and animals. (2006)
Journal Article
Kroon, J., Wei, W., Simon, J., & Slabas, A. (2006). Identification and functional expression of a type 2 acyl-CoA :diacylglycerol acyltransferase (DGAT2) in developing castor bean seedswhich has high homology to the major triglyceride biosynthetic enzymeof fungi and animals. Phytochemistry, 67(23), 2541-2549