Outputs (37)

Inhibition of PDIs Downregulates Core LINC Complex Proteins, Promoting the Invasiveness of MDA-MB-231 Breast Cancer Cells in Confined Spaces In Vitro (2024)
Journal Article

Eukaryotic cells tether the nucleoskeleton to the cytoskeleton via a conserved molecular bridge, called the LINC complex. The core of the LINC complex comprises SUN-domain and KASH-domain proteins that directly associate within the nuclear envelope l... Read More about Inhibition of PDIs Downregulates Core LINC Complex Proteins, Promoting the Invasiveness of MDA-MB-231 Breast Cancer Cells in Confined Spaces In Vitro.

An Intronic Heterozygous SYNE2 Splice Site Mutation: A Rare Cause for Myalgia and hyperCKemia? (2024)
Journal Article

SYNE2 mutations have been associated with skeletal and cardiac muscle diseases, including Emery-Dreifuss muscular dystrophy (EDMD). Here, we present a 70-year-old male patient with muscle pain and elevated serum creatine kinase levels in whom whole-e... Read More about An Intronic Heterozygous SYNE2 Splice Site Mutation: A Rare Cause for Myalgia and hyperCKemia?.

Quantitative morphometric analysis of intrinsic and extrinsic skin ageing in individuals with Fitzpatrick skin types II–III (2023)
Journal Article

Skin ageing is an intricate physiological process affected by intrinsic and extrinsic factors. There is a demand to understand how the skin changes with age and photoexposure in individuals with Fitzpatrick skin types I-III due to accelerated photoag... Read More about Quantitative morphometric analysis of intrinsic and extrinsic skin ageing in individuals with Fitzpatrick skin types II–III.

Biallelic SYNE2 Missense Mutations Leading to Nesprin-2 Giant Hypo-Expression Are Associated with Intellectual Disability and Autism (2021)
Journal Article

Autism spectrum disorder (ASD) is a group of neurological and developmental disabilities characterised by clinical and genetic heterogeneity. The current study aimed to expand ASD genotyping by investigating potential associations with SYNE2 mutation... Read More about Biallelic SYNE2 Missense Mutations Leading to Nesprin-2 Giant Hypo-Expression Are Associated with Intellectual Disability and Autism.

Culturing Keratinocytes on Biomimetic Substrates Facilitates Improved Epidermal Assembly In Vitro (2021)
Journal Article

Mechanotransduction is defined as the ability of cells to sense mechanical stimuli from their surroundings and translate them into biochemical signals. Epidermal keratinocytes respond to mechanical cues by altering their proliferation, migration, and... Read More about Culturing Keratinocytes on Biomimetic Substrates Facilitates Improved Epidermal Assembly In Vitro.

Bioengineering the Microanatomy of Human Skin (2019)
Journal Article

Recreating the structure of human tissues in the laboratory is valuable for fundamental research, testing interventions, and reducing the use of animals. Critical to the use of such technology is the ability to produce tissue models that accurately r... Read More about Bioengineering the Microanatomy of Human Skin.

Functional Analysis of LINC Complexes in the Skin (2018)
Book Chapter

The genome in eukaryotic cells is encased by two intricate and interconnected concentric membranes, which together with the underlying nuclear lamina form the nuclear envelope (NE). Two fundamental macromolecular structures are embedded within the nu... Read More about Functional Analysis of LINC Complexes in the Skin.

p63 transcription factor regulates nuclear shape and expression of nuclear envelope-associated genes in epidermal keratinocytes (2017)
Journal Article

The maintenance of a proper nuclear architecture and 3D organization of the genes, enhancer elements and transcription machinery plays an essential role in tissue development and regeneration. Here we show that in the developing skin, epidermal proge... Read More about p63 transcription factor regulates nuclear shape and expression of nuclear envelope-associated genes in epidermal keratinocytes.

Detection of Diverse and High Molecular Weight Nesprin-1 and Nesprin-2 Isoforms Using Western Blotting (2016)
Book Chapter

Heavily utilized in cell and molecular biology, western blotting is considered a crucial technique for the detection and quantification of proteins within complex mixtures. In particular, the detection of members of the nesprin (nuclear envelope spec... Read More about Detection of Diverse and High Molecular Weight Nesprin-1 and Nesprin-2 Isoforms Using Western Blotting.

Nesprins in health and disease. (2013)
Journal Article

LINC (Linker of Nucleoskeleton and Cytoskeleton) complex is an evolutionary conserved structure that spans the entire nuclear envelope (NE), and integrates the nuclear interior with the cytoskeleton, in order to support a diverse array of fundamental... Read More about Nesprins in health and disease..

Roles for nuclear envelope associated Nesprin-2 Giant in cell differentiation, proliferation and chromatin association in a mouse model. (2012)
Journal Article

LINC complex alterations in DMD and EDMD/CMT fibroblasts. (2012)
Journal Article

Nesprin interchain associations control nuclear size. (2012)
Journal Article

Nesprins-1/-2/-3/-4 are nuclear envelope proteins, which connect nuclei to the cytoskeleton. The largest nesprin-1/-2 isoforms (termed giant) tether F-actin through their N-terminal actin binding domain (ABD). Nesprin-3, however, lacks an ABD and ass... Read More about Nesprin interchain associations control nuclear size..

Cytotoxic T lymphocyte effector function is independent of nucleus–centrosome dissociation (2012)
Journal Article

Cytotoxic T lymphocytes (CTLs) kill tumorigenic and virally infected cells by targeted secretion of lytic granule contents. The precise point at which secretion occurs is directed by the centrosome docking at the immunological synapse (IS). The centr... Read More about Cytotoxic T lymphocyte effector function is independent of nucleus–centrosome dissociation.

Cytoskeletal interactions at the nuclear envelope mediated by Nesprins (2012)
Journal Article

Nesprin-1 is a giant tail-anchored nuclear envelope protein composed of an N-terminal F-actin binding domain, a long linker region formed by multiple spectrin repeats and a C-terminal transmembrane domain. Based on this structure, it connects the nuc... Read More about Cytoskeletal interactions at the nuclear envelope mediated by Nesprins.

Molecular mechanisms of centrosome and cytoskeleton anchorage at the nuclear envelope. (2011)
Journal Article

Nesprin-2 interacts with α-Catenin and regulates Wnt signaling at the nuclear envelope. (2010)
Journal Article

Induction of a massive endoplasmic reticulum and perinuclear space expansion by expression of lamin B receptor mutants and the related sterol reductases TM7SF2 and DHCR7. (2010)
Journal Article

The LINC-less granulocyte nucleus (2009)
Journal Article

The major blood granulocyte (neutrophil) is rapidly recruited to sites of bacterial and fungal infections. It is a highly malleable cell, allowing it to squeeze out of blood vessels and migrate through tight tissue spaces. The human granulocyte nucle... Read More about The LINC-less granulocyte nucleus.

Nesprin-2 Giant (NUANCE) maintains nuclear envelope architecture and composition in skin (2008)
Journal Article

Giant isoforms, encoded by Nesprin-1 (Syne1) and Nesprin-2 (Syne2), are multifunctional actin-binding and nuclear-envelope-associated proteins belonging to the spectrin superfamily. Here, we investigate the function of Nesprin-2 Giant (NUANCE) in ski... Read More about Nesprin-2 Giant (NUANCE) maintains nuclear envelope architecture and composition in skin.

Sun1 forms immobile macromolecular assemblies at the nuclear envelope (2008)
Journal Article

SUN-domain proteins form a novel and conserved family of inner nuclear membrane (INM) proteins, which establish physical connections between the nucleoplasm and the cytoskeleton. In the current study, we provide evidence that within the nuclear envel... Read More about Sun1 forms immobile macromolecular assemblies at the nuclear envelope.

KASH-domain proteins and the cytoskeletal landscapes of the nuclear envelope (2008)
Journal Article

Over the last few years, several novel proteins have been identified that facilitate the physical integration of the nucleus with the cytoplasmic compartment. The majority belong to the evolutionarily conserved KASH [klarsicht/ANC-1 (anchorage 1)/SYN... Read More about KASH-domain proteins and the cytoskeletal landscapes of the nuclear envelope.

Delta6-desaturase (FADS2) deficiency unveils the role of omega3- and omega6-polyunsaturated fatty acids (2008)
Journal Article

Mammalian cell viability is dependent on the supply of the essential fatty acids (EFAs) linoleic and alpha-linolenic acid. EFAs are converted into omega3- and omega6-polyunsaturated fatty acids (PUFAs), which are essential constituents of membrane ph... Read More about Delta6-desaturase (FADS2) deficiency unveils the role of omega3- and omega6-polyunsaturated fatty acids.

CAP2, cyclase-associated protein 2, is a dual compartment protein (2007)
Journal Article

Cyclase-associated proteins (CAPs) are evolutionarily conserved proteins with roles in regulating the actin cytoskeleton and in signal transduction. Mammals have two CAP genes encoding the related CAP1 and CAP2. We studied the distribution and subcel... Read More about CAP2, cyclase-associated protein 2, is a dual compartment protein.

Nesprin-2 giant safeguards nuclear envelope architecture in LMNA S143F progeria cells (2007)
Journal Article

The S143F lamin A/C point mutation causes a phenotype combining features of myopathy and progeria. We demonstrate here that patient dermal fibroblast cells have dysmorphic nuclei containing numerous blebs and lobulations, which progressively accumula... Read More about Nesprin-2 giant safeguards nuclear envelope architecture in LMNA S143F progeria cells.

The inner nuclear membrane protein Sun1 mediates the anchorage of Nesprin-2 to the nuclear envelope (2005)
Journal Article

Nesprins form a novel class of nuclear envelope-anchored spectrin-repeat proteins. We show that a direct association of their highly conserved C-terminal luminal domain with the inner nuclear membrane protein Sun1 mediates their nuclear envelope loca... Read More about The inner nuclear membrane protein Sun1 mediates the anchorage of Nesprin-2 to the nuclear envelope.

Lamin A/C-dependent localization of Nesprin-2, a giant scaffolder at the nuclear envelope (2005)
Journal Article

The vertebrate proteins Nesprin-1 and Nesprin-2 (also referred to as Enaptin and NUANCE) together with ANC-1 of Caenorhabditis elegans and MSP-300 of Drosophila melanogaster belong to a novel family of -actinin type actin-binding proteins residing at... Read More about Lamin A/C-dependent localization of Nesprin-2, a giant scaffolder at the nuclear envelope.

Enaptin, a giant actin-binding protein, is an element of the nuclear membrane and the actin cytoskeleton (2004)
Journal Article

Enaptin belongs to a family of recently identified giant proteins that associate with the F-actin cytoskeleton as well as the nuclear membrane. It is composed of an N-terminal alpha-actinin type actin-binding domain (ABD) followed by a long coiled co... Read More about Enaptin, a giant actin-binding protein, is an element of the nuclear membrane and the actin cytoskeleton.

ACF7: An essential integrator of microtubule dynamics (2003)
Journal Article

ACF7 is a member of the spectraplakin family of cytoskeletal crosslinking proteins possessing actin and microtubule binding domains. Here, we show that ACF7 is an essential integrator of MT-actin dynamics. In endodermal cells, ACF7 binds along microt... Read More about ACF7: An essential integrator of microtubule dynamics.

Bridging cytoskeletal intersections. (2001)
Journal Article

An epidermal plakin that integrates actin and microtubule networks at cellular junctions. (2000)
Journal Article

Dissection of functional domains by expression of point-mutated profilins in Dictyostelium mutants. (2000)
Journal Article

Identification of a suppressor of the Dictyostelium profilin-minus phenotype as a CD36/LIMP-II homologue. (1999)
Journal Article

Plant profilins rescue the aberrant phenotype of profilin-deficient Dictyostelium cells. (1996)
Journal Article

Identification of a cyclase-associated protein (CAP) homologue in Dictyostelium discoideum and characterization of its interaction with actin (1996)
Journal Article

In search for novel actin binding proteins in Dictyostelium discoideum we have isolated a cDNA clone coding for a protein of approximately 50 kDa that is highly homologous to the class of adenylyl cyclase-associated proteins (CAP). In Saccharomyces c... Read More about Identification of a cyclase-associated protein (CAP) homologue in Dictyostelium discoideum and characterization of its interaction with actin.

Structure/function studies on cytoskeletal proteins in Dictyostelium amoebae as a paradigm (1995)
Journal Article

The actin cytoskeleton in motile non-muscle cells is being regulated by a large number of actin-binding proteins. A deeper insight into the complex nature of the dynamic rearrangements of the microfilament system during cell movement requires an expe... Read More about Structure/function studies on cytoskeletal proteins in Dictyostelium amoebae as a paradigm.

Dictyostelium amoebae that lack G-actin-sequestering profilins show defects in F-actin content, cytokinesis, and development (1994)
Journal Article

To study in vivo functions of the ubiquitous actin-binding protein profilin, we generated by antisense and gene disruption techniques Dictyostelium mutants that lack one or both of the profilin isoforms. Whereas the single mutants showed an essential... Read More about Dictyostelium amoebae that lack G-actin-sequestering profilins show defects in F-actin content, cytokinesis, and development.