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Functional Analysis of LINC Complexes in the Skin

Karakesisoglou, Iakowos; Mroß, Carmen; Noegel, Angelika A.


Carmen Mroß

Angelika A. Noegel


Gregg G. Gundersen

Howard J. Worman


The genome in eukaryotic cells is encased by two intricate and interconnected concentric membranes, which together with the underlying nuclear lamina form the nuclear envelope (NE). Two fundamental macromolecular structures are embedded within the nuclear envelope: the nuclear pore (NPC) and the LINC complex. The former perforates the nucleus controlling biomolecule trafficking between the nucleoplasm and the cytoplasm, while the latter integrates the nucleus via the cytoskeleton to the extracellular matrix. LINC complex structural and functional integrity is of utmost importance for various fundamental cellular functions. Mechanical forces are relayed into the nuclear interior via the LINC complex, which controls lamina organization, chromosome dynamics, and genome organization and stability. Thus, LINC constituents play pivotal roles in cellular architecture including organelle positioning, cell movement, tissue assembly, organ homeostasis, and organismal aging. The LINC complex oligomeric core contains several multi-isomeric, multifunctional, and often tissue-specific proteins. Therefore, for a proper functional analysis, genetic mouse models are an invaluable resource. Herein, we focus on the LINC complex roles in the skin and describe methods that enable the successful isolation of primary embryonic fibroblast and newborn skin cells, which can be then investigated functionally in vitro.


Karakesisoglou, I., Mroß, C., & Noegel, A. A. (2018). Functional Analysis of LINC Complexes in the Skin. In G. G. Gundersen, & H. J. Worman (Eds.), The LINC Complex: Methods and Protocols (295-306).

Online Publication Date Aug 23, 2018
Publication Date Aug 24, 2018
Deposit Date Feb 6, 2020
Pages 295-306
Series Title Methods in Molecular Biology
Series Number 1840
Series ISSN 1064-3745
Book Title The LINC Complex: Methods and Protocols
ISBN 9781493986903
Public URL