Dr Gary Sharples' Outputs (2)

Structural and functional characterization of the Redβ recombinase from bacteriophage λ (2013)
Journal Article
Matsubara, K., Malay, A., Curtis, F., Sharples, G., & Heddle, J. (2013). Structural and functional characterization of the Redβ recombinase from bacteriophage λ. PLoS ONE, 8(11), Article e78869. https://doi.org/10.1371/journal.pone.0078869

The Red system of bacteriophage λ is responsible for the genetic rearrangements that contribute to its rapid evolution and has been successfully harnessed as a research tool for genome manipulation. The key recombination component is Redβ, a ring-sha... Read More about Structural and functional characterization of the Redβ recombinase from bacteriophage λ.

Mutants of phage bIL67 RuvC with enhanced Holliday junction binding selectivity and resolution symmetry (2013)
Journal Article
Green, V., Curtis, F., Sedelnikova, S., Rafferty, J., & Sharples, G. (2013). Mutants of phage bIL67 RuvC with enhanced Holliday junction binding selectivity and resolution symmetry. Molecular Microbiology, 89(6), 1240-1258. https://doi.org/10.1111/mmi.12343

Viral and bacterial Holliday junction resolvases differ in specificity with the former typically being more promiscuous, acting on a variety of branched DNA substrates, while the latter exclusively targets Holliday junctions. We have determined the c... Read More about Mutants of phage bIL67 RuvC with enhanced Holliday junction binding selectivity and resolution symmetry.