All Outputs (143)

Early onset of senescence and imbalanced epidermal homeostasis across the decades in photoexposed human skin: Fingerprints of inflammaging (2022)
Journal Article
Jarrold, B. B., Tan, C. Y. R., Ho, C. Y., Soon, A. L., Lam, T. T., Yang, X., Nguyen, C., Guo, W., Chew, Y. C., DeAngelis, Y. M., Costello, L., De Los Santos Gomez, P., Przyborski, S., Bellanger, S., Dreesen, O., Kimball, A. B., & Oblong, J. E. (2022). Early onset of senescence and imbalanced epidermal homeostasis across the decades in photoexposed human skin: Fingerprints of inflammaging. Experimental Dermatology, 31(11), 1748-1760. https://doi.org/10.1111/exd.14654

Inflammaging is a theory of ageing which purports that low-level chronic inflammation leads to cellular dysfunction and premature ageing of surrounding tissue. Skin is susceptible to inflammaging because it is the first line of defence from the envir... Read More about Early onset of senescence and imbalanced epidermal homeostasis across the decades in photoexposed human skin: Fingerprints of inflammaging.

Recreating Tissue Structures Representative of Teratomas In Vitro Using a Combination of 3D Cell Culture Technology and Human Embryonic Stem Cells (2022)
Journal Article
Hidalgo Aguilar, A., Smith, L., Owens, D., Quelch, R., & Przyborski, S. (2022). Recreating Tissue Structures Representative of Teratomas In Vitro Using a Combination of 3D Cell Culture Technology and Human Embryonic Stem Cells. Bioengineering, 9(5), Article 185. https://doi.org/10.3390/bioengineering9050185

In vitro studies using human embryonic stem cells (hESCs) are a valuable method to study aspects of embryogenesis, avoiding ethical issues when using embryonic materials and species dissimilarities. The xenograft teratoma assay is often traditionally... Read More about Recreating Tissue Structures Representative of Teratomas In Vitro Using a Combination of 3D Cell Culture Technology and Human Embryonic Stem Cells.

Tissue engineering strategies to bioengineer the ageing skin phenotype in vitro (2022)
Journal Article
Costello, L., Dicolandrea, T., Tasseff, R., Isfort, R., Bascom, C., von Zglinicki, T., & Przyborski, S. (2022). Tissue engineering strategies to bioengineer the ageing skin phenotype in vitro. Aging Cell, 21(2), Article e13550. https://doi.org/10.1111/acel.13550

Human skin ageing is a complex and heterogeneous process, which is influenced by genetically determined intrinsic factors and accelerated by cumulative exposure to extrinsic stressors. In the current world ageing demographic, there is a requirement f... Read More about Tissue engineering strategies to bioengineer the ageing skin phenotype in vitro.

Development of a mammalian neurosensory full‐thickness skin equivalent and its application to screen sensitizing stimuli (2022)
Journal Article
Freer, M., Darling, N., Goncalves, K., Mills, K. J., & Przyborski, S. (2023). Development of a mammalian neurosensory full‐thickness skin equivalent and its application to screen sensitizing stimuli. Bioengineering and Translational Medicine, 8(3), Article e10484. https://doi.org/10.1002/btm2.10484

Human skin equivalents (HSEs) are an increasingly popular research tool due to limitations associated with animal testing for dermatological research. They recapitulate many aspects of skin structure and function, however, many only contain two basic... Read More about Development of a mammalian neurosensory full‐thickness skin equivalent and its application to screen sensitizing stimuli.

How good is the evidence that cellular senescence causes skin ageing? (2021)
Journal Article
Low, E., Alimohammadiha, G., Smith, L. A., Costello, L. F., Przyborski, S. A., von Zglinicki, T., & Miwa, S. (2021). How good is the evidence that cellular senescence causes skin ageing?. Ageing Research Reviews, 71, https://doi.org/10.1016/j.arr.2021.101456

Skin is the largest organ of the body with important protective functions, which become compromised with time due to both intrinsic and extrinsic ageing processes. Cellular senescence is the primary ageing process at cell level, associated with loss... Read More about How good is the evidence that cellular senescence causes skin ageing?.

The Cell-Permeable Derivative of the Immunoregulatory Metabolite Itaconate, 4-Octyl Itaconate, Is Anti-Fibrotic in Systemic Sclerosis (2021)
Journal Article
Henderson, J., Dayalan Naidu, S., Dinkova-Kostova, A. T., Przyborski, S., Stratton, R., & O′Reilly, S. (2021). The Cell-Permeable Derivative of the Immunoregulatory Metabolite Itaconate, 4-Octyl Itaconate, Is Anti-Fibrotic in Systemic Sclerosis. Cells, 10(8), https://doi.org/10.3390/cells10082053

Systemic sclerosis (SSc) is an autoimmune connective tissue disease that leads to skin fibrosis. Altered metabolism has recently been described in autoimmune diseases and SSc. Itaconate is a product of the Krebs cycle intermediate cis-aconitate and i... Read More about The Cell-Permeable Derivative of the Immunoregulatory Metabolite Itaconate, 4-Octyl Itaconate, Is Anti-Fibrotic in Systemic Sclerosis.

Bone Morphogenetic Protein Antagonist Gremlin-1 Increases Myofibroblast Transition in Dermal Fibroblasts: Implications for Systemic Sclerosis (2021)
Journal Article
Duffy, L., Henderson, J., Brown, M., Pryzborski, S., Fullard, N., Summa, L., Distler, J. H., Stratton, R., & O’Reilly, S. (2021). Bone Morphogenetic Protein Antagonist Gremlin-1 Increases Myofibroblast Transition in Dermal Fibroblasts: Implications for Systemic Sclerosis. Frontiers in Cell and Developmental Biology, 9, Article 681061. https://doi.org/10.3389/fcell.2021.681061

Objective: Systemic Sclerosis is an autoimmune connective tissue disease which results in fibrosis of the skin and lungs. The disease is characterized by activation of myofibroblasts but what governs this is unknown. Gremlin-1 is a BMP antagonist tha... Read More about Bone Morphogenetic Protein Antagonist Gremlin-1 Increases Myofibroblast Transition in Dermal Fibroblasts: Implications for Systemic Sclerosis.

Using Advanced Cell Culture Techniques to Differentiate Pluripotent Stem Cells and Recreate Tissue Structures Representative of Teratoma Xenografts (2021)
Journal Article
Smith, L., Hidalgo Aguilar, A., Owens, D., Quelch, R., Knight, E., & Przyborski, S. (2021). Using Advanced Cell Culture Techniques to Differentiate Pluripotent Stem Cells and Recreate Tissue Structures Representative of Teratoma Xenografts. Frontiers in Cell and Developmental Biology, 9, https://doi.org/10.3389/fcell.2021.667246

Various methods are currently used to investigate human tissue differentiation, including human embryo culture and studies utilising pluripotent stem cells (PSCs) such as in vitro embryoid body formation and in vivo teratoma assays. Each method has i... Read More about Using Advanced Cell Culture Techniques to Differentiate Pluripotent Stem Cells and Recreate Tissue Structures Representative of Teratoma Xenografts.

The vitamin A ester retinyl propionate has a unique metabolic profile and higher retinoid‐related bioactivity over retinol and retinyl palmitate in human skin models (2020)
Journal Article
Bjerke, D. L., Li, R., Price, J. M., Dobson, R. L., Rodrigues, M., Tey, C., Vires, L., Adams, R. L., Sherrill, J. D., Styczynski, P. B., Goncalves, K., Maltman, V., Przyborski, S., & Oblong, J. E. (2021). The vitamin A ester retinyl propionate has a unique metabolic profile and higher retinoid‐related bioactivity over retinol and retinyl palmitate in human skin models. Experimental Dermatology, 30(2), 226-236. https://doi.org/10.1111/exd.14219

Human skin is exposed daily to environmental stressors, which cause acute damage and inflammation. Over time this leads to morphological and visual appearance changes associated with premature aging. Topical vitamin A derivatives such as retinol (ROL... Read More about The vitamin A ester retinyl propionate has a unique metabolic profile and higher retinoid‐related bioactivity over retinol and retinyl palmitate in human skin models.

microRNA27a-3p mediates reduction of the Wnt antagonist sFRP-1 in systemic sclerosis (2020)
Journal Article
Henderson, J., Wilkinson, S., Przyborski, S., Stratton, R., & O’Reilly, S. (2021). microRNA27a-3p mediates reduction of the Wnt antagonist sFRP-1 in systemic sclerosis. Epigenetics, 16(7), 808-817. https://doi.org/10.1080/15592294.2020.1827715

Systemic Sclerosis (SSc) is an autoimmune connective tissue disease that leads to skin and lung fibrosis. The Wnt pathway is clearly elevated in SSc and is pro-fibrotic via activation of canonical Wnt signalling. sFRP-1 is a Wnt antagonist that acts... Read More about microRNA27a-3p mediates reduction of the Wnt antagonist sFRP-1 in systemic sclerosis.

Bioengineering Novel in vitro Co-culture Models That Represent the Human Intestinal Mucosa With Improved Caco-2 Structure and Barrier Function (2020)
Journal Article
Darling, N. J., Mobbs, C. L., González-Hau, A. L., Freer, M., & Przyborski, S. (2020). Bioengineering Novel in vitro Co-culture Models That Represent the Human Intestinal Mucosa With Improved Caco-2 Structure and Barrier Function. Frontiers in Bioengineering and Biotechnology, 8, Article 992. https://doi.org/10.3389/fbioe.2020.00992

The Caco-2 monolayer is the most widely used in vitro model of the human intestinal mucosa to study absorption. However, models lack communication from other cells present in the native intestine, such as signals from fibroblasts in the lamina propri... Read More about Bioengineering Novel in vitro Co-culture Models That Represent the Human Intestinal Mucosa With Improved Caco-2 Structure and Barrier Function.

Germline TET2 loss of function causes childhood immunodeficiency and lymphoma (2020)
Journal Article
Stremenova Spegarova, J., Lawless, D., Mohamad, S. M. B., Engelhardt, K. R., Doody, G., Shrimpton, J., Rensing-Ehl, A., Ehl, S., Rieux-Laucat, F., Cargo, C., Griffin, H., Mikulasova, A., Acres, M., Morgan, N. V., Poulter, J. A., Sheridan, E. G., Chetcuti, P., O'Riordan, S., Anwar, R., Carter, C. R., …Hambleton, S. (2020). Germline TET2 loss of function causes childhood immunodeficiency and lymphoma. Blood, 136(9), 1055-1066. https://doi.org/10.1182/blood.2020005844

Molecular dissection of inborn errors of immunity can help to elucidate the nonredundant functions of individual genes. We studied 3 children with an immune dysregulation syndrome of susceptibility to infection, lymphadenopathy, hepatosplenomegaly, d... Read More about Germline TET2 loss of function causes childhood immunodeficiency and lymphoma.

Multiple scattering in scanning helium microscopy (2020)
Journal Article
Lambrick, S., Vozdecký, L., Bergin, M., Halpin, J., MacLaren, D., Dastoor, P., (Przyborski, S., Jardine, A., & Ward, D. (2020). Multiple scattering in scanning helium microscopy. Applied Physics Letters, 116(6), Article 061601. https://doi.org/10.1063/1.5143950

Using atom beams to image the surface of samples in real space is an emerging technique that delivers unique contrast from delicate samples. Here, we explore the contrast that arises from multiple scattering of helium atoms, a specific process that p... Read More about Multiple scattering in scanning helium microscopy.

Engineering a Multilayered Skin Equivalent: The Importance of Endogenous Extracellular Matrix Maturation to Provide Robustness and Reproducibility (2019)
Book Chapter
Costello, L., Fullard, N., Roger, M., Bradbury, S., Dicolandrea, T., Isfort, R., …Przyborski, S. (2019). Engineering a Multilayered Skin Equivalent: The Importance of Endogenous Extracellular Matrix Maturation to Provide Robustness and Reproducibility. In S. Böttcher-Haberzeth, & T. Biedermann (Eds.), Skin Tissue Engineering (107-122). https://doi.org/10.1007/978-1-4939-9473-1_9

Bioengineering the Microanatomy of Human Skin (2019)
Journal Article
Roger, M., Fullard, N., Costello, L., Bradbury, S., Markiewicz, E., O'Reilly, S., Darling, N., Ritchie, P., Määttä, A., Karakesisoglou, I., Nelson, G., von Zglinicki, T., Dicolandrea, T., Isfort, R., Bascom, C., & Przyborski, S. (2019). Bioengineering the Microanatomy of Human Skin. Journal of Anatomy, 234(4), 438-455. https://doi.org/10.1111/joa.12942

Recreating the structure of human tissues in the laboratory is valuable for fundamental research, testing interventions, and reducing the use of animals. Critical to the use of such technology is the ability to produce tissue models that accurately r... Read More about Bioengineering the Microanatomy of Human Skin.

An essential role for the Zn2+ transporter ZIP7 in B cell development (2019)
Journal Article
Anzilotti, C., Swan, D. J., Boisson, B., Deobagkar-Lele, M., Oliveira, C., Chabosseau, P., Engelhardt, K. R., Xu, X., Chen, R., Alvarez, L., Berlinguer-Palmini, R., Bull, K. R., Cawthorne, E., Cribbs, A. P., Crockford, T. L., Dang, T. S., Fearn, A., Fenech, E. J., de Jong, S. J., Lagerholm, B., …Hambleton, S. (2019). An essential role for the Zn2+ transporter ZIP7 in B cell development. Nature Immunology, 20, 350-361. https://doi.org/10.1038/s41590-018-0295-8

Despite the known importance of zinc for human immunity, molecular insights into its roles have remained limited. Here we report a novel autosomal recessive disease characterized by absent B cells, agammaglobulinemia and early onset infections in fiv... Read More about An essential role for the Zn2+ transporter ZIP7 in B cell development.

Disrupted alternative splicing for genes implicated in splicing and ciliogenesis causes PRPF31 retinitis pigmentosa (2018)
Journal Article
Buskin, A., Zhu, L., Chichagova, V., Basu, B., Mozaffari-Jovin, S., Dolan, D., Droop, A., Collin, J., Bronstein, R., Mehrotra, S., Farkas, M., Hilgen, G., White, K., Pan, K.-T., Treumann, A., Hallam, D., Bialas, K., Chung, G., Mellough, C., Ding, Y., …Lako, M. (2018). Disrupted alternative splicing for genes implicated in splicing and ciliogenesis causes PRPF31 retinitis pigmentosa. Nature Communications, 9(1), Article 4234. https://doi.org/10.1038/s41467-018-06448-y

Mutations in pre-mRNA processing factors (PRPFs) cause autosomal-dominant retinitis pigmentosa (RP), but it is unclear why mutations in ubiquitously expressed genes cause non-syndromic retinal disease. Here, we generate transcriptome profiles from RP... Read More about Disrupted alternative splicing for genes implicated in splicing and ciliogenesis causes PRPF31 retinitis pigmentosa.

PyCoTools: a Python toolbox for COPASI (2018)
Journal Article
Welsh, C. M., Fullard, N., Proctor, C. J., Martinez-Guimera, A., Isfort, R. J., Bascom, C. C., Tasseff, R., Przyborski, S. A., & Shanley, D. P. (2018). PyCoTools: a Python toolbox for COPASI. Bioinformatics, 34(21), 3702-3710. https://doi.org/10.1093/bioinformatics/bty409

Motivation COPASI is an open source software package for constructing, simulating and analyzing dynamic models of biochemical networks. COPASI is primarily intended to be used with a graphical user interface but often it is desirable to be able to ac... Read More about PyCoTools: a Python toolbox for COPASI.

iPSC modeling of severe aplastic anemia reveals impaired differentiation and telomere shortening in blood progenitors (2018)
Journal Article
Melguizo-Sanchis, D., Xu, Y., Taheem, D., Yu, M., Tilgner, K., Barta, T., …Lako, M. (2018). iPSC modeling of severe aplastic anemia reveals impaired differentiation and telomere shortening in blood progenitors. Cell Death and Disease, 9(2), Article 128. https://doi.org/10.1038/s41419-017-0141-1

Aplastic Anemia (AA) is a bone marrow failure (BMF) disorder, resulting in bone marrow hypocellularity and peripheral pancytopenia. Severe aplastic anemia (SAA) is a subset of AA defined by a more severe phenotype. Although the immunological nature o... Read More about iPSC modeling of severe aplastic anemia reveals impaired differentiation and telomere shortening in blood progenitors.

An iPSC Patient Specific Model of CFH (Y402H) Polymorphism Displays Characteristic Features of AMD and Indicates a Beneficial Role for UV Light Exposure (2017)
Journal Article
Hallam, D., Collin, J., Bojic, S., Chichagova, V., Buskin, A., Xu, Y., …Lako, M. (2017). An iPSC Patient Specific Model of CFH (Y402H) Polymorphism Displays Characteristic Features of AMD and Indicates a Beneficial Role for UV Light Exposure. Stem Cells, 35(11), 2305-2320. https://doi.org/10.1002/stem.2708

Age related macular degeneration (AMD) is the most common cause of blindness, accounting for 8.7% of all blindness globally. Vision loss is caused ultimately by apoptosis of the retinal pigment epithelium (RPE) and overlying photoreceptors. Treatment... Read More about An iPSC Patient Specific Model of CFH (Y402H) Polymorphism Displays Characteristic Features of AMD and Indicates a Beneficial Role for UV Light Exposure.