Global low-frequency motions in protein allostery: CAP as a model system

Townsend, P.D.; Rogers, T.L.; Pohl, E.; Wilson, M.R.; McLeish, T.C.B.; Cann, M.J.

doi:10.1007/s12551-015-0163-9

A Non-Functional Carbon Dioxide-Mediated Post-Translational Modification on Nucleoside Diphosphate Kinase of Arabidopsis thaliana (2024)
Journal Article
Gannon, H. G., Riaz-Bradley, A., & Cann, M. J. (2024). A Non-Functional Carbon Dioxide-Mediated Post-Translational Modification on Nucleoside Diphosphate Kinase of Arabidopsis thaliana. International Journal of Molecular Sciences, 25(2), Article 898. https://doi.org/10.3390/ijms25020898

The carbamate post-translational modification (PTM), formed by the nucleophilic attack of carbon dioxide by a dissociated lysine epsilon-amino group, is proposed as a widespread mechanism for sensing this biologically important bioactive gas. Here, w... Read More about A Non-Functional Carbon Dioxide-Mediated Post-Translational Modification on Nucleoside Diphosphate Kinase of Arabidopsis thaliana.

Near atmospheric carbon dioxide activates plant ubiquitin cross-linking (2023)
Journal Article
Gannon, H. G., & Cann, M. J. (2023). Near atmospheric carbon dioxide activates plant ubiquitin cross-linking. BBA Advances, 4, Article 100096. https://doi.org/10.1016/j.bbadva.2023.100096

Background Identifying CO2-binding proteins is vital for our knowledge of CO2-regulated molecular processes. The carbamate post-translational modification is a reversible CO2-mediated adduct that can form on neutral N-terminal α-amino or lysine ε-ami... Read More about Near atmospheric carbon dioxide activates plant ubiquitin cross-linking.

Allophycocyanin A is a carbon dioxide receptor in the cyanobacterial phycobilisome (2022)
Journal Article
Guillen-Garcia, A., Gibson, S., Jordan, C., Ramaswamy, V., Linthwaite, V., Bromley, E., Brown, A., Hodgson, D., Blower, T., Verlet, J., Degiacomi, M., Palsson, L.-O., & Cann, M. (2022). Allophycocyanin A is a carbon dioxide receptor in the cyanobacterial phycobilisome. Nature Communications, 13, Article 5289. https://doi.org/10.1038/s41467-022-32925-6

Light harvesting is fundamental for production of ATP and reducing equivalents for CO2 fixation during photosynthesis. However, electronic energy transfer (EET) through a photosystem can harm the photosynthetic apparatus when not balanced with CO2. H... Read More about Allophycocyanin A is a carbon dioxide receptor in the cyanobacterial phycobilisome.

Carbon Dioxide and the Carbamate Post-Translational Modification (2022)
Journal Article
Blake, L. I., & Cann, M. J. (2022). Carbon Dioxide and the Carbamate Post-Translational Modification. Frontiers in Molecular Biosciences, 9, Article 825706. https://doi.org/10.3389/fmolb.2022.825706

Carbon dioxide is essential for life. It is at the beginning of every life process as a substrate of photosynthesis. It is at the end of every life process as the product of post-mortem decay. Therefore, it is not surprising that this gas regulates s... Read More about Carbon Dioxide and the Carbamate Post-Translational Modification.

Ubiquitin is a carbon dioxide-binding protein (2021)
Journal Article
Linthwaite, V., Pawloski, W., Pegg, H., Townsend, P., Thomas, M., Brown, A., Hodgson, D., Lorimer, G., Fushman, D., & Cann, M. (2021). Ubiquitin is a carbon dioxide-binding protein. Science Advances, 7(39), Article eabi5507. https://doi.org/10.1126/sciadv.abi5507

The identification of CO2-binding proteins is crucial to understanding CO2-regulated molecular processes. CO2 can form a reversible posttranslational modification through carbamylation of neutral N-terminal -amino or lysine -amino groups. We have p... Read More about Ubiquitin is a carbon dioxide-binding protein.

Applying TADF Emitters in Bioimaging and Sensing—A Novel Approach Using Liposomes for Encapsulation and Cellular Uptake (2021)
Journal Article
Smith, P. O., Black, D. J., Pal, R., Avó, J., Dias, F. B., Linthwaite, V. L., Cann, M. J., & Pålsson, L.-O. (2021). Applying TADF Emitters in Bioimaging and Sensing—A Novel Approach Using Liposomes for Encapsulation and Cellular Uptake. Frontiers in Chemistry, 9, Article 743928. https://doi.org/10.3389/fchem.2021.743928

A new method for facilitating the delivery, uptake and intracellular localisation of thermally activated delayed fluorescence (TADF) complexes was developed. First, confinement of TADF complexes in liposomes was demonstrated, which were subsequently... Read More about Applying TADF Emitters in Bioimaging and Sensing—A Novel Approach Using Liposomes for Encapsulation and Cellular Uptake.

A methodology for carbamate post-translational modification discovery and its application in Escherichia coli (2021)
Journal Article
Linthwaite, V. L., & Cann, M. J. (2021). A methodology for carbamate post-translational modification discovery and its application in Escherichia coli. Interface Focus, 11(2), Article 20200028. https://doi.org/10.1098/rsfs.2020.0028

Carbon dioxide can influence cell phenotypes through the modulation of signalling pathways. CO2 regulates cellular processes as diverse as metabolism, cellular homeostasis, chemosensing and pathogenesis. This diversity of regulated processes suggests... Read More about A methodology for carbamate post-translational modification discovery and its application in Escherichia coli.

Opposing modulation of Cx26 gap junctions and hemichannels by CO2 (2020)
Journal Article
Nijjar, S., Maddison, D., Meigh, L., Wolf, E., Rodgers, T., Cann, M., & Dale, N. (2021). Opposing modulation of Cx26 gap junctions and hemichannels by CO2. The Journal of Physiology, 599(1), 103-118. https://doi.org/10.1113/jp280747

Cx26 hemichannels open in response to moderate elevations of CO2 (PCO2 55 mmHg) via a carbamylation reaction that depends on residues K125 and R104. Here we investigate the action of CO2 on Cx26 gap junctions. Using a dye transfer assay, we found tha... Read More about Opposing modulation of Cx26 gap junctions and hemichannels by CO2.

A DNA-binding bromodomain-containing protein interacts with and reduces Rx1-mediated immune response to Potato Virus X (2020)
Journal Article
Sukarta, O. C., Townsend, P. D., Llewelyn, A., Dixon, C. H., Slootweg, E. J., Pålsson, L.-O., Takken, F. L., Goverse, A., & Cann, M. J. (2020). A DNA-binding bromodomain-containing protein interacts with and reduces Rx1-mediated immune response to Potato Virus X. Plant communications, 1(4), Article 100086. https://doi.org/10.1016/j.xplc.2020.100086

Plant NLR proteins enable the immune system to recognise and respond to pathogen attack. An early consequence of immune activation is transcriptional reprogramming. Some NLRs have been shown to act in the nucleus and interact with transcription facto... Read More about A DNA-binding bromodomain-containing protein interacts with and reduces Rx1-mediated immune response to Potato Virus X.

Distinct Roles of Non-Overlapping Surface Regions of the Coiled-Coil Domain in the Potato Immune Receptor Rx1 (2018)
Journal Article
Slootweg, E. J., Spiridon, L. N., Martin, E. C., Tameling, W. I., Townsend, P. D., Pomp, R., Roosien, J., Drawska, O., Sukarta, O. C., Schots, A., Borst, J. W., Joosten, M. H., Bakker, J., Smant, G., Cann, M. J., Petrescu, A.-J., & Goverse, A. (2018). Distinct Roles of Non-Overlapping Surface Regions of the Coiled-Coil Domain in the Potato Immune Receptor Rx1. Plant Physiology, 178(3), 1310-1331. https://doi.org/10.1104/pp.18.00603

The intracellular immune receptor Rx1 of potato (Solanum tuberosum), which confers effector-triggered immunity to Potato virus X, consists of a central nucleotide-binding domain (NB-ARC) flanked by a carboxyl-terminal leucine-rich repeat (LRR) domain... Read More about Distinct Roles of Non-Overlapping Surface Regions of the Coiled-Coil Domain in the Potato Immune Receptor Rx1.

The identification of carbon dioxide mediated protein post-translational modifications (2018)
Journal Article
Linthwaite, V., Janus, J., Brown, A., Wong-Pascua, D., O’Donoghue, A., Porter, A., Treumann, A., Hodgson, D., & Cann, M. (2018). The identification of carbon dioxide mediated protein post-translational modifications. Nature Communications, 9, Article 3092. https://doi.org/10.1038/s41467-018-05475-z

Carbon dioxide is vital to the chemistry of life processes including metabolism, cellular homoeostasis, and pathogenesis. CO2 is generally unreactive but can combine with neutral amines to form carbamates on proteins under physiological conditions. T... Read More about The identification of carbon dioxide mediated protein post-translational modifications.

The intracellular immune receptor Rx1 regulates the DNA-binding activity of a Golden2-like transcription factor (2017)
Journal Article
Townsend, P., Dixon, C., Slootweg, E., Sukarta, O., Yang, A., Hughes, T., …Cann, M. (2018). The intracellular immune receptor Rx1 regulates the DNA-binding activity of a Golden2-like transcription factor. Journal of Biological Chemistry, 293(9), 3218-3233. https://doi.org/10.1074/jbc.ra117.000485

Plant NLR proteins enable the immune system to recognise and respond to pathogen attack. An early consequence of immune activation is transcriptional reprogramming and some NLRs have been shown to act in the nucleus and interact with transcription fa... Read More about The intracellular immune receptor Rx1 regulates the DNA-binding activity of a Golden2-like transcription factor.

Hypercapnia modulates cAMP signalling and cystic fibrosis transmembrane conductance regulator-dependent anion and fluid secretion in airway epithelia (2015)
Journal Article
Turner, M., Saint-Crib, V., Patel, W., Ibrahim, S., Verdon, B., Ward, C., …Gray, M. (2016). Hypercapnia modulates cAMP signalling and cystic fibrosis transmembrane conductance regulator-dependent anion and fluid secretion in airway epithelia. The Journal of Physiology, 594(6), 1643-1661. https://doi.org/10.1113/jp271309

Hypercapnia is clinically defined as an arterial blood partial pressure of CO2 of above 40 mmHg and is a feature of chronic lung disease. In previous studies we have demonstrated that hypercapnia modulates agonist-stimulated cAMP levels through effec... Read More about Hypercapnia modulates cAMP signalling and cystic fibrosis transmembrane conductance regulator-dependent anion and fluid secretion in airway epithelia.

The tomato Nucleotide-Binding Leucine-Rich Repeat (NLR) Immune Receptor I-2 couples DNA-Binding to Nucleotide-Binding Domain Nucleotide Exchange (2015)
Journal Article
Fenyk, S., Dixon, C. H., Kittens, W. H., Townsend, P. D., Sharples, G. .., Pålsson, L. .-O., Takken, F. L. W., & Cann, M. J. (2016). The tomato Nucleotide-Binding Leucine-Rich Repeat (NLR) Immune Receptor I-2 couples DNA-Binding to Nucleotide-Binding Domain Nucleotide Exchange. Journal of Biological Chemistry, 291(3), 1137-1147. https://doi.org/10.1074/jbc.m115.698589

Plant nucleotide-binding leucine-rich repeat (NLR) proteins enable plants to recognise and respond to pathogen attack. Previously, we demonstrated that the Rx1 NLR of potato is able to bind and bend DNA in vitro. DNA binding in situ requires its genu... Read More about The tomato Nucleotide-Binding Leucine-Rich Repeat (NLR) Immune Receptor I-2 couples DNA-Binding to Nucleotide-Binding Domain Nucleotide Exchange.

Multi-scale Approaches to Dynamical Transmission of Protein Allostery (2015)
Presentation / Conference Contribution
Townsend, P., Rodgers, T., Pohl, E., Wilson, M., Cann, M., & McLeish, T. (2015, January). Multi-scale Approaches to Dynamical Transmission of Protein Allostery. Presented at Physical Biology of Proteins and Peptides, Mexico City, Mexico

We review the idea that allosteric interactions can be transmitted not by structural switching but by the more subtle route of modulation of the amplitude of thermally-activated global dynamical modes in allosteric proteins. The effect is naturally a... Read More about Multi-scale Approaches to Dynamical Transmission of Protein Allostery.

Dynamic Transmission of Protein Allostery without Structural Change: Spatial Pathways or Global Modes? (2015)
Journal Article
McLeish, T., Cann, M., & Rodgers, T. (2015). Dynamic Transmission of Protein Allostery without Structural Change: Spatial Pathways or Global Modes?. Biophysical Journal, 109(6), 1240-1250. https://doi.org/10.1016/j.bpj.2015.08.009

We examine the contrast between mechanisms for allosteric signaling that involve structural change, and those that do not, from the perspective of allosteric pathways. In particular we treat in detail the case of fluctuation-allostery by which amplit... Read More about Dynamic Transmission of Protein Allostery without Structural Change: Spatial Pathways or Global Modes?.

The Potato Nucleotide-Binding Leucine-Rich Repeat (NLR) Immune Receptor Rx1 is a Pathogen Dependent DNA-Deforming Protein (2015)
Journal Article
Fenyk, S., Townsend, P. D., Dixon, C. H., Spies, G. B., de San Eustaquio Campillo, A., Slootweg, E. J., …Cann, M. J. (2015). The Potato Nucleotide-Binding Leucine-Rich Repeat (NLR) Immune Receptor Rx1 is a Pathogen Dependent DNA-Deforming Protein. Journal of Biological Chemistry, 290(41), 24945-24960. https://doi.org/10.1074/jbc.m115.672121

Plant NLR proteins enable cells to respond to pathogen attack. Several NLRs act in the nucleus, however, conserved nuclear targets that support their role in immunity are unknown. Previously we noted a structural homology between the NB domain of NLR... Read More about The Potato Nucleotide-Binding Leucine-Rich Repeat (NLR) Immune Receptor Rx1 is a Pathogen Dependent DNA-Deforming Protein.

Ecto-5'-Nucleotidase, Adenosine and Transmembrane Adenylyl Cyclase Signalling Regulate Basal Carotid Body Chemoafferent Outflow and Establish the Sensitivity to Hypercapnia. (2015)
Book Chapter
Holmes, A., Nunes, A., Cann, M., & Kumar, P. (2015). Ecto-5'-Nucleotidase, Adenosine and Transmembrane Adenylyl Cyclase Signalling Regulate Basal Carotid Body Chemoafferent Outflow and Establish the Sensitivity to Hypercapnia. In C. Peers, P. Kumar, C. Wyatt, E. Gauda, C. A. Nurse, & N. Prabhakar (Eds.), Arterial Chemoreceptors in Physiology and Pathophysiology (279-289). Springer. https://doi.org/10.1007/978-3-319-18440-1_32

Carotid body (CB) stimulation by hypercapnia causes a reflex increase in ventilation and, along with the central chemoreceptors, this prevents a potentially lethal systemic acidosis. Control over the CB chemoafferent output during normocapnia and hyp... Read More about Ecto-5'-Nucleotidase, Adenosine and Transmembrane Adenylyl Cyclase Signalling Regulate Basal Carotid Body Chemoafferent Outflow and Establish the Sensitivity to Hypercapnia..

The role of protein-ligand contacts in allosteric regulation of the Escherichia coli Catabolite Activator Protein (2015)
Journal Article
Townsend, P., Rodgers, T., Glover, L., Korhonen, H., Richards, S., Colwell, L., …Cann, M. (2015). The role of protein-ligand contacts in allosteric regulation of the Escherichia coli Catabolite Activator Protein. Journal of Biological Chemistry, 290(36), 22225-22235. https://doi.org/10.1074/jbc.m115.669267

Allostery is a fundamental process by which ligand binding to a protein alters its activity at a distant site. Both experimental and theoretical evidence demonstrate that allostery can be communicated through altered slow relaxation protein dynamics... Read More about The role of protein-ligand contacts in allosteric regulation of the Escherichia coli Catabolite Activator Protein.

Global low-frequency motions in protein allostery: CAP as a model system (2015)
Journal Article
Townsend, P., Rogers, T., Pohl, E., Wilson, M., McLeish, T., & Cann, M. (2015). Global low-frequency motions in protein allostery: CAP as a model system. Biophysical Reviews, 7(2), 175-182. https://doi.org/10.1007/s12551-015-0163-9

Allostery is a fundamental process by which ligand binding to a protein alters its activity at a distant site. There is considerable evidence that allosteric cooperativity can be communicated by the modulation of protein dynamics without conformation... Read More about Global low-frequency motions in protein allostery: CAP as a model system.

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