All Outputs (26)

Three-dimensional data capture and analysis of intact eye lenses evidences emmetropia-associated changes and strain-dependent differences in epithelial cell organization (2020)
Journal Article
Kalligeraki, A. A., Isted, A., Pal, R., Saunter, C., Girkin, J., Jarrin, M., Uwineza, A., Obara, B., & Quinlan, R. A. (2020). Three-dimensional data capture and analysis of intact eye lenses evidences emmetropia-associated changes and strain-dependent differences in epithelial cell organization. Scientific Reports, 10, Article 16898. https://doi.org/10.1038/s41598-020-73625-9

Organ and tissue development are highly coordinated processes; lens growth and functional integration into the eye (emmetropia) is a robust example. An epithelial monolayer covers the anterior hemisphere of the lens, and its organization is the key t... Read More about Three-dimensional data capture and analysis of intact eye lenses evidences emmetropia-associated changes and strain-dependent differences in epithelial cell organization.

Endothelial TRPV4 channels modulate vascular tone by Ca2+‐induced Ca2+ release at inositol 1,4,5‐trisphosphate receptors (2019)
Journal Article
Heathcote, H. R., Lee, M. D., Zhang, X., Saunter, C. D., Wilson, C., & McCarron, J. G. (2019). Endothelial TRPV4 channels modulate vascular tone by Ca2+‐induced Ca2+ release at inositol 1,4,5‐trisphosphate receptors. British Journal of Pharmacology, 176(17), 3297-3317. https://doi.org/10.1111/bph.14762

Background and Purpose: The TRPV4 ion channels are Ca2+ permeable, non‐selective cation channels that mediate large, but highly localized, Ca2+ signals in the endothelium. The mechanisms that permit highly localized Ca2+ changes to evoke cell‐wide ac... Read More about Endothelial TRPV4 channels modulate vascular tone by Ca2+‐induced Ca2+ release at inositol 1,4,5‐trisphosphate receptors.

Generating artificial reference images for open loop correlation wavefront sensors (2018)
Journal Article
Townson, M., Love, G., & Saunter, C. (2018). Generating artificial reference images for open loop correlation wavefront sensors. Monthly Notices of the Royal Astronomical Society, 479(2), 1595-1602. https://doi.org/10.1093/mnras/sty1362

Shack–Hartmann wavefront sensors for both solar and laser guide star adaptive optics (with elongated spots) need to observe extended objects. Correlation techniques have been successfully employed to measure the wavefront gradient in solar adaptive o... Read More about Generating artificial reference images for open loop correlation wavefront sensors.

Non-invasive in vivo quantification of the developing optical properties and graded index of the embryonic eye lens using SPIM (2018)
Journal Article
Young, L., Jarrin, M., Saunter, C., Quinlan, R., & Girkin, J. (2018). Non-invasive in vivo quantification of the developing optical properties and graded index of the embryonic eye lens using SPIM. Biomedical Optics Express, 9(5), 2176-2188. https://doi.org/10.1364/boe.9.002176

Graded refractive index lenses are inherent to advanced visual systems in animals. By understanding their formation and local optical properties, significant potential for improved ocular healthcare may be realized. We report a novel technique measur... Read More about Non-invasive in vivo quantification of the developing optical properties and graded index of the embryonic eye lens using SPIM.

Advancing Age Decreases Pressure-Sensitive Modulation of Calcium Signaling in the Endothelium of Intact and Pressurized Arteries (2017)
Journal Article
Wilson, C., Saunter, C. D., Girkin, J. M., & McCarron, J. G. (2017). Advancing Age Decreases Pressure-Sensitive Modulation of Calcium Signaling in the Endothelium of Intact and Pressurized Arteries. Journal of Vascular Research, 53(5-6), 358-369. https://doi.org/10.1159/000454811

Aging is the summation of many subtle changes which result in altered cardiovascular function. Impaired endothelial function underlies several of these changes and precipitates plaque development in larger arteries. The endothelium transduces chemica... Read More about Advancing Age Decreases Pressure-Sensitive Modulation of Calcium Signaling in the Endothelium of Intact and Pressurized Arteries.

Reducing the Field of View in Correlating Wavefront Sensors for Solar Adaptive Optics (2017)
Presentation / Conference Contribution
Townson, M. J., & Saunter, C. D. (2017, June). Reducing the Field of View in Correlating Wavefront Sensors for Solar Adaptive Optics. Presented at Adaptive Optics for Extremely Large Telescopes 5 (AO4ELT5), Tenerife, Canary Islands, Spain

Wavefront sensing for solar adaptive optics currently requires the use of extended-field Shack-Hartmann wavefront sensors. Such wavefront sensors suffer from a reduced sensitivity to high altitude turbulence due effects of measuring wavefront gradien... Read More about Reducing the Field of View in Correlating Wavefront Sensors for Solar Adaptive Optics.

Age decreases mitochondrial motility and increases mitochondrial size in vascular smooth muscle (2016)
Journal Article
Chalmers, S., Saunter, C., Girkin, J., & McCarron, J. (2016). Age decreases mitochondrial motility and increases mitochondrial size in vascular smooth muscle. The Journal of Physiology, 594(15), 4283-4295. https://doi.org/10.1113/jp271942

Mitochondrial function, motility and architecture are each central to cell function. Age-associated mitochondrial dysfunction may contribute to vascular disease. However, mitochondrial changes in ageing remain ill-defined because of the challenges of... Read More about Age decreases mitochondrial motility and increases mitochondrial size in vascular smooth muscle.

Clusters of specialized detector cells provide sensitive and high fidelity receptor signaling in the intact endothelium (2016)
Journal Article
Wilson, C., Saunter, C. D., Girkin, J. M., & McCarron, J. G. (2016). Clusters of specialized detector cells provide sensitive and high fidelity receptor signaling in the intact endothelium. FASEB Journal, 30(5), 2000-2013. https://doi.org/10.1096/fj.201500090

Agonist-mediated signaling by the endothelium controls virtually all vascular functions. Because of the large diversity of agonists, each with varying concentrations, background noise often obscures individual cellular signals. How the endothelium di... Read More about Clusters of specialized detector cells provide sensitive and high fidelity receptor signaling in the intact endothelium.

Flicker-assisted localization microscopy reveals altered mitochondrial architecture in hypertension (2015)
Journal Article
Chalmers, S., Saunter, C. D., Girkin, J. M., & McCarron, J. G. (2015). Flicker-assisted localization microscopy reveals altered mitochondrial architecture in hypertension. Scientific Reports, 5, Article 16875. https://doi.org/10.1038/srep16875

Mitochondrial morphology is central to normal physiology and disease development. However, in many live cells and tissues, complex mitochondrial structures exist and morphology has been difficult to quantify. We have measured the shape of electricall... Read More about Flicker-assisted localization microscopy reveals altered mitochondrial architecture in hypertension.

Pressure-dependent regulation of Ca2+ signalling in the vascular endothelium (2015)
Journal Article
Wilson, C., Saunter, C. D., Girkin, J. M., & McCarron, J. G. (2015). Pressure-dependent regulation of Ca2+ signalling in the vascular endothelium. The Journal of Physiology, 593(24), 5231-5253. https://doi.org/10.1113/jp271157

The endothelium is an interconnected network upon which haemodynamic mechanical forces act to control vascular tone and remodelling in disease. Ca2+ signalling is central to the endothelium's mechanotransduction and networked activity. However, chall... Read More about Pressure-dependent regulation of Ca2+ signalling in the vascular endothelium.

Improved shift estimates on extended Shack–Hartmann wavefront sensor images (2015)
Journal Article
Townson, M., Kellerer, A., & Saunter, C. (2015). Improved shift estimates on extended Shack–Hartmann wavefront sensor images. Monthly Notices of the Royal Astronomical Society, 452(4), 4022-4028. https://doi.org/10.1093/mnras/stv1503

An important factor which affects performance of solar adaptive optics (AO) systems is the accuracy of tracking an extended object in the wavefront sensor. The accuracy of a centre-of-mass approach to image shift measurement depends on the parameters... Read More about Improved shift estimates on extended Shack–Hartmann wavefront sensor images.

Quantitative High Dynamic Range Beam Proling for Fluorescence Microscopy (2014)
Journal Article
Mitchell, T., Saunter, C., O'Nions, W., Girkin, J., & Love, G. (2014). Quantitative High Dynamic Range Beam Proling for Fluorescence Microscopy. Review of Scientific Instruments, 85(10), Article 103713. https://doi.org/10.1063/1.4899208

Modern developmental biology relies on optically-sectioning uorescence microscope techniques to produce non-destructive in-vivo images of developing specimens at high resolution in three dimensions. As optimal performance of these techniques is relia... Read More about Quantitative High Dynamic Range Beam Proling for Fluorescence Microscopy.

Combining optical trapping in a microfluidic channel with simultaneous micro-Raman spectroscopy and motion detection (2014)
Presentation / Conference Contribution
Lawton, P., Saunter, C., & Girkin, J. (2014, March). Combining optical trapping in a microfluidic channel with simultaneous micro-Raman spectroscopy and motion detection. Presented at Microfluidics, BioMEMS, and Medical Microsystems XII, San Francisco, California, United States

Since their invention by Ashkin optical tweezers have demonstrated their ability and versatility as a non-invasive tool for micromanipulation. One of the most useful additions to the basic optical tweezers system is micro-Raman spectroscopy, which pe... Read More about Combining optical trapping in a microfluidic channel with simultaneous micro-Raman spectroscopy and motion detection.

AO modal optimization in a live, beating zebrafish heart (2013)
Presentation / Conference Contribution
Bourgenot, C., Taylor, J. M., Saunter, C. D., Girkin, J. M., & Love, G. D. (2013, June). AO modal optimization in a live, beating zebrafish heart. Presented at Imaging and Applied Optics Postdeadline, Arlington, Virginia, United States

We report on the combinated use of heart synchronization and wavefront sensorless modal AO to enable significant improvement in image quality of a live transgenic zebrafish embryo heart, imaged with a light sheet microscope.

Comparison of closed loop and sensorless adaptive optics in widefield optical microscopy (2013)
Journal Article
Bourgenot, C., Saunter, C., Love, G., & Girkin, J. (2013). Comparison of closed loop and sensorless adaptive optics in widefield optical microscopy. Journal of the European Optical Society-Rapid Publications, 8, Article 13027. https://doi.org/10.2971/jeos.2013.13027

We report on a closed loop widefield adaptive optics, optical microscopy system in which the feedback signal is provided by backscattered light from the sample acting as a guide star. The improvement in imaging performance is compared to an adaptive... Read More about Comparison of closed loop and sensorless adaptive optics in widefield optical microscopy.

Light sheet adaptive optics microscope for 3D live imaging (2013)
Presentation / Conference Contribution
Bourgenot, C., Taylor, J., Saunter, C., Girkin, J., & Love, G. (2013, February). Light sheet adaptive optics microscope for 3D live imaging. Presented at Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX, San Francisco, California, USA

We report on the incorporation of adaptive optics (AO) into the imaging arm of a selective plane illumination microscope (SPIM). SPIM has recently emerged as an important tool for life science research due to its ability to deliver high-speed, optica... Read More about Light sheet adaptive optics microscope for 3D live imaging.

Adaptive optical methods for in vivo imaging in developing Zebra fish (2012)
Presentation / Conference Contribution
Girkin, J., Taylor, J., Bourgenot, C., Saunter, C., & Love, G. (2012, December). Adaptive optical methods for in vivo imaging in developing Zebra fish. Presented at 2012 International Symposium on Optomechatronic Technologies (ISOT 2012), Paris

The humble Zebra fish is rapidly establishing itself as the model of choice for a wide range of biological investigations, in particular at the developing embryo stage. Single Plane Illumination Microscopy (SPIM) has already been shown to be a very p... Read More about Adaptive optical methods for in vivo imaging in developing Zebra fish.

3D adaptive optics in a light sheet microscope (2012)
Journal Article
Bourgenot, C., Saunter, C. D., Taylor, J. M., Girkin, J. M., & Love, G. D. (2012). 3D adaptive optics in a light sheet microscope. Optics Express, 20(12), 13252-13261. https://doi.org/10.1364/oe.20.013252

We report on a single plane illumination microscope (SPIM) incorporating adaptive optics in the imaging arm. We show how aberrations can occur from the sample mounting tube and quantify the aberrations both experimentally and computationally. A wavef... Read More about 3D adaptive optics in a light sheet microscope.

Closed loop adaptive optics with a laser guide star for biological light microscopy (2012)
Presentation / Conference Contribution
Saunter, C. D., Bourgenot, C., Girkin, J. M., & Love, G. D. (2012, January). Closed loop adaptive optics with a laser guide star for biological light microscopy. Presented at MEMS Adaptive Optics VI, San Francisco, CA

We report on the development of a widefield microscope that achieves adaptive optics correction through the use of a wavefront sensor observing an artificial laser guide star induced within the sample. By generating this guide star at arbitrary posit... Read More about Closed loop adaptive optics with a laser guide star for biological light microscopy.

Wide field adaptive optics microscopy using both closed loop correction and image sharpness optimization (2011)
Presentation / Conference Contribution
Bourgenot, C., Love, G. D., Saunter, C. D., & Girkin, J. M. (2011, July). Wide field adaptive optics microscopy using both closed loop correction and image sharpness optimization. Presented at Imaging and Applied Optics, Toronto, Canada

We report on results from a wide field microscope fitted with adaptive optics. We describe results based on both image optimization (wavefront sensorless adaptive optics) and full closed loop correction.