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3D adaptive optics in a light sheet microscope

Bourgenot, Cyril; Saunter, Christopher D.; Taylor, Jonathan M.; Girkin, John M.; Love, Gordon D.

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Authors

Christopher D. Saunter

Jonathan M. Taylor

Gordon D. Love



Abstract

We report on a single plane illumination microscope (SPIM) incorporating adaptive optics in the imaging arm. We show how aberrations can occur from the sample mounting tube and quantify the aberrations both experimentally and computationally. A wavefront sensorless approach was taken to imaging a green fluorescent protein (GFP) labelled transgenic zebrafish. We show improvements in image quality whilst recording a 3D “z–stack” and show how the aberrations come from varying depths in the fish.

Citation

Bourgenot, C., Saunter, C. D., Taylor, J. M., Girkin, J. M., & Love, G. D. (2012). 3D adaptive optics in a light sheet microscope. Optics Express, 20(12), 13252-13261. https://doi.org/10.1364/oe.20.013252

Journal Article Type Article
Publication Date Jun 1, 2012
Deposit Date May 22, 2014
Publicly Available Date Jul 29, 2014
Journal Optics Express
Electronic ISSN 1094-4087
Publisher Optica
Peer Reviewed Peer Reviewed
Volume 20
Issue 12
Article Number 13252-13261
Pages 13252-13261
DOI https://doi.org/10.1364/oe.20.013252
Public URL https://durham-repository.worktribe.com/output/1454338

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Published Journal Article (1.8 Mb)
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Copyright Statement
This paper was published in Optics Express and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: http://dx.doi.org/10.1364/OE.20.013252. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.






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