All Outputs (4)

Characterization of Proteins Localized to Plant ER-PM Contact Sites (2017)
Book Chapter
Wang, P., Hawes, C., Richardson, C., & Hussey, P. J. (2018). Characterization of Proteins Localized to Plant ER-PM Contact Sites. In C. Hawes, & V. Kriechbaumer (Eds.), The Plant Endoplasmic Reticulum (23-31). Humana Press. https://doi.org/10.1007/978-1-4939-7389-7_3

Like in most eukaryotic cells, the plant endoplasmic reticulum (ER) network is physically linked to the plasma membrane (PM), forming ER-PM contact sites (EPCS). The protein complex required for maintaining the EPCS is composed of ER integral membran... Read More about Characterization of Proteins Localized to Plant ER-PM Contact Sites.

Immunoelectron Microscopy of Cryofixed Freeze-Substituted Yeast (2016)
Book Chapter
Fišerová, J., Richardson, C., & Goldberg, M. W. (2016). Immunoelectron Microscopy of Cryofixed Freeze-Substituted Yeast. In S. D. Schwartzbach, O. Skalli, & T. Schikorski (Eds.), High-Resolution Imaging of Cellular Proteins (243-258). Humana Press. https://doi.org/10.1007/978-1-4939-6352-2_15

Immunolabeling electron microscopy is a challenging technique with demands for perfect ultrastructural and antigen preservation. High-pressure freezing offers an excellent way to fix cellular structure. However, its use for immunolabeling has remaine... Read More about Immunoelectron Microscopy of Cryofixed Freeze-Substituted Yeast.

Imaging Yeast NPCs: From Classical Electron Microscopy to Immuno-SEM (2014)
Book Chapter
Kiseleva, E., Richardson, A. C., Fiserova, J., Strunov, A. A., Spink, M. C., Johnson, S. R., & Goldberg, M. W. (2014). Imaging Yeast NPCs: From Classical Electron Microscopy to Immuno-SEM. In Nuclear Pore Complexes and Nucleocytoplasmic Transport - Methods (59-79). Elsevier/Academic Press. https://doi.org/10.1016/b978-0-12-417160-2.00003-5

Electron microscopy (EM) has been used extensively for the study of nuclear transport as well as the structure of the nuclear pore complex (NPC) and nuclear envelope. However, there are specific challenges faced when carrying out EM in one of the mai... Read More about Imaging Yeast NPCs: From Classical Electron Microscopy to Immuno-SEM.

Evidence for activity-dependent modulation of sensory-terminal excitability in spindles by glutamate release from synaptic-like vesicles (2002)
Book Chapter
Banks, R., Bewick, G., Reid, B., & Richardson, C. (2002). Evidence for activity-dependent modulation of sensory-terminal excitability in spindles by glutamate release from synaptic-like vesicles. In S. Gandevia, U. Proske, & D. Stuart (Eds.), Sensorimotor control of movement and posture (13-18). (2002). Springer Verlag. https://doi.org/10.1007/978-1-4615-0713-0_2

Sensory terminals of muscle spindles and similar mechanosensory neurons contain large numbers of 50 nm, "synaptic-like" vesicles (SLVs), about whose role very little is known. Using fluorescence microscopy, immunocytochemistry and electrophysiologica... Read More about Evidence for activity-dependent modulation of sensory-terminal excitability in spindles by glutamate release from synaptic-like vesicles.