Functional networks of inhibitory neurons orchestrate synchrony in the hippocampus

Bocchio, Marco; Vorobyev, Artem; Sadeh, Sadra; Brustlein, Sophie; Dard, Robin; Reichinnek, Susanne; Emiliani, Valentina; Baude, Agnes; Clopath, Claudia; Cossart, Rosa

Abstract

Inhibitory interneurons are pivotal components of cortical circuits. Beyond providing inhibition, they have been proposed to coordinate the firing of excitatory neurons within cell assemblies. While the roles of specific interneuron subtypes have been extensively studied, their influence on pyramidal cell synchrony in vivo remains elusive. Employing an all-optical approach in mice, we simultaneously recorded hippocampal interneurons and pyramidal cells and probed the network influence of individual interneurons using optogenetics. We demonstrate that CA1 interneurons form a functionally interconnected network that promotes synchrony through disinhibition during awake immobility, while preserving endogenous cell assemblies. Our network model underscores the importance of both cell assemblies and dense, unspecific interneuron connectivity in explaining our experimental findings, suggesting that interneurons may operate not only via division of labor but also through concerted activity.

Citation

Bocchio, M., Vorobyev, A., Sadeh, S., Brustlein, S., Dard, R., Reichinnek, S., Emiliani, V., Baude, A., Clopath, C., & Cossart, R. (2024). Functional networks of inhibitory neurons orchestrate synchrony in the hippocampus. PLoS Biology, 22(10), Article e3002837. https://doi.org/10.1371/journal.pbio.3002837

• Journal Article Type: Article
• Acceptance Date: Sep 6, 2024
• Online Publication Date: Oct 14, 2024
• Publication Date: 2024-10
• Deposit Date: Nov 1, 2024
• Publicly Available Date: Nov 1, 2024
• Journal: PLOS Biology
• Print ISSN: 1544-9173
• Electronic ISSN: 1545-7885
• Publisher: Public Library of Science
• Peer Reviewed: Peer Reviewed
• Volume: 22
• Issue: 10
• Article Number: e3002837
• DOI: https://doi.org/10.1371/journal.pbio.3002837
• Public URL: https://durham-repository.worktribe.com/output/2989588