Genetic and epigenetic reprogramming in response to internal and external cues by induced transposon mobilization in Moso bamboo

Zou, Long-Hai; Zhu, Bailiang; Chen, Yaxin; Lu, Yaping; Ramkrishnan, Muthusamy; Xu, Chao; Zhou, Xiaohong; Ding, Yiqian; Cho, Jungnam; Zhou, Mingbing

Abstract

Summary Long terminal repeat retroelements (LTR-REs) have profound effects on DNA methylation and gene regulation. Despite the vast abundance of LTR-REs in the genome of Moso bamboo (Phyllostachys edulis), an industrial crop in underdeveloped countries, their precise implication of the LTR-RE mobility in stress response and development remains unknown. We investigated the RNA and DNA products of LTR-REs in Moso bamboo under various developmental stages and stressful conditions. Surprisingly, our analyses identified thousands of active LTR-REs, particularly those located near genes involved in stress response and developmental regulation. These genes adjacent to active LTR-REs exhibited an increased expression under stress and are associated with reduced DNA methylation that is likely affected by the induced LTR-REs. Moreover, the analyses of simultaneous mapping of insertions and DNA methylation showed that the LTR-REs effectively alter the epigenetic status of the genomic regions where they inserted, and concomitantly their transcriptional competence which might impact the stress resilience and growth of the host. Our work unveils the unusually strong LTR-RE mobility in Moso bamboo and its close association with (epi)genetic changes, which supports the co-evolution of the parasitic DNAs and host genome in attaining stress tolerance and developmental robustness.

Citation

Zou, L.-H., Zhu, B., Chen, Y., Lu, Y., Ramkrishnan, M., Xu, C., Zhou, X., Ding, Y., Cho, J., & Zhou, M. (2024). Genetic and epigenetic reprogramming in response to internal and external cues by induced transposon mobilization in Moso bamboo. New Phytologist, 244(5), 1916-1930. https://doi.org/10.1111/nph.20107

• Journal Article Type: Article
• Acceptance Date: Aug 21, 2024
• Online Publication Date: Sep 5, 2024
• Publication Date: 2024-12
• Deposit Date: Sep 6, 2024
• Publicly Available Date: Sep 11, 2024
• Journal: New Phytologist
• Print ISSN: 0028-646X
• Electronic ISSN: 1469-8137
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 244
• Issue: 5
• Pages: 1916-1930
• DOI: https://doi.org/10.1111/nph.20107
• Keywords: DNA methylation, ALE‐seq, eclDNA, Phyllostachys edulis, LTR retrotransposons
• Public URL: https://durham-repository.worktribe.com/output/2799070