Exo84c-regulated degradation is involved in the normal self-incompatible response in Brassicaceae

Zhang, Tong; Wang, Kun; Dou, Shengwei; Gao, Erlin; Hussey, Patrick J; Lin, Zongcheng; Wang, Pengwei

Abstract

The self-incompatibility system evolves in angiosperms to promote cross-pollination by rejecting self-pollination. Here, we show the involvement of Exo84c in the SI response of both Brassica napus and Arabidopsis. The expression of Exo84c is specifically elevated in stigma during the SI response. Knocking out Exo84c in B.napus and SI Arabidopsis partially breaks down the SI response. The SI response inhibits both the protein secretion in papillae and the recruitment of the exocyst complex to the pollen-pistil contact sites. Interestingly, these processes can be partially restored in exo84c SI Arabidopsis. After incompatible pollination, the turnover of the exocyst-labeled compartment is enhanced in papillae. However, this process is perturbed in exo84c SI Arabidopsis. Taken together, our results suggest that Exo84c regulates the exocyst complex vacuolar degradation during the SI response. This process is likely independent of the known SI pathway in Brassicaceae to secure the SI response. [Abstract copyright: Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.]

Citation

Zhang, T., Wang, K., Dou, S., Gao, E., Hussey, P. J., Lin, Z., & Wang, P. (2024). Exo84c-regulated degradation is involved in the normal self-incompatible response in Brassicaceae. Cell Reports, 43(3), Article 113913. https://doi.org/10.1016/j.celrep.2024.113913

• Journal Article Type: Article
• Acceptance Date: Feb 19, 2024
• Online Publication Date: Mar 4, 2024
• Publication Date: Mar 26, 2024
• Deposit Date: Mar 14, 2024
• Publicly Available Date: Mar 15, 2024
• Journal: Cell Reports
• Print ISSN: 2211-1247
• Publisher: Cell Press
• Peer Reviewed: Peer Reviewed
• Volume: 43
• Issue: 3
• Article Number: 113913
• DOI: https://doi.org/10.1016/j.celrep.2024.113913
• Keywords: self-incompatibility, autophagy, degradation, exocyst complex, live cell imaging, secretion, CP: Cell biology, Brassicaceae, CP: Plants
• Public URL: https://durham-repository.worktribe.com/output/2328667