Molecular Characterization and Genome Mechanical Features of Two Newly Isolated Polyvalent Bacteriophages Infecting Pseudomonas syringae pv. garcae

Silva, Erica C; Quinde, Carlos A; Cieza, Basilio; Basu, Aakash; Vila, Marta M D C; Balcão, Victor M

Abstract

Coffee plants have been targeted by a devastating bacterial disease, a condition known as bacterial blight, caused by the phytopathogen Pseudomonas syringae pv. garcae (Psg). Conventional treatments of coffee plantations affected by the disease involve frequent spraying with copper- and kasugamycin-derived compounds, but they are both highly toxic to the environment and stimulate the appearance of bacterial resistance. Herein, we report the molecular characterization and mechanical features of the genome of two newly isolated (putative polyvalent) lytic phages for Psg. The isolated phages belong to class Caudoviricetes and present a myovirus-like morphotype belonging to the genuses Tequatrovirus (PsgM02F) and Phapecoctavirus (PsgM04F) of the subfamilies Straboviridae (PsgM02F) and Stephanstirmvirinae (PsgM04F), according to recent bacterial viruses' taxonomy, based on their complete genome sequences. The 165,282 bp (PsgM02F) and 151,205 bp (PsgM04F) genomes do not feature any lysogenic-related (integrase) genes and, hence, can safely be assumed to follow a lytic lifestyle. While phage PsgM02F produced a morphogenesis yield of 124 virions per host cell, phage PsgM04F produced only 12 virions per host cell, indicating that they replicate well in Psg with a 50 min latency period. Genome mechanical analyses established a relationship between genome bendability and virion morphogenesis yield within infected host cells.

Citation

Silva, E. C., Quinde, C. A., Cieza, B., Basu, A., Vila, M. M. D. C., & Balcão, V. M. (2024). Molecular Characterization and Genome Mechanical Features of Two Newly Isolated Polyvalent Bacteriophages Infecting Pseudomonas syringae pv. garcae. Genes, 15(1), Article 113. https://doi.org/10.3390/genes15010113

• Journal Article Type: Article
• Acceptance Date: Jan 16, 2024
• Online Publication Date: Jan 18, 2024
• Publication Date: 2024-01
• Deposit Date: Mar 25, 2024
• Publicly Available Date: Mar 25, 2024
• Journal: Genes
• Electronic ISSN: 2073-4425
• Publisher: MDPI
• Peer Reviewed: Peer Reviewed
• Volume: 15
• Issue: 1
• Article Number: 113
• DOI: https://doi.org/10.3390/genes15010113
• Public URL: https://durham-repository.worktribe.com/output/2192770
• PMID: 38255005