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Spider venom neurotoxin based bioinsecticides: A novel bioactive for the control of the Asian citrus psyllid Diaphorina citri (Hemiptera)

Miranda, Marcelo P; Fitches, Elaine C; Sukiran, Nur Afiqah; Eduardo, Wellington I; Garcia, Rafael B; Jaciani, Fabrício J; Readshaw, Jennifer J; Bell, Jack; Peña, Leandro

Spider venom neurotoxin based bioinsecticides: A novel bioactive for the control of the Asian citrus psyllid Diaphorina citri (Hemiptera) Thumbnail


Authors

Marcelo P Miranda

Wellington I Eduardo

Rafael B Garcia

Fabrício J Jaciani

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Jenny Readshaw jennifer.readshaw@durham.ac.uk
PGR Student Doctor of Philosophy

Dr Jack Bell jack.a.bell@durham.ac.uk
Post Doctoral Research Associate

Leandro Peña



Abstract

The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae), is a key vector of the phloem-limited bacteria Candidatus Liberibacter asiaticus (CLas) associated with huanglongbing (HLB), the most serious and currently incurable disease of citrus worldwide. Here we report the first investigation into the potential use of a spider venom-derived recombinant neurotoxin, ω/κ-HxTx-Hv1h (hereafter HxTx-Hv1h) when delivered alone or when fused to snowdrop lectin (Galanthus nivalis agglutinin; GNA) to control D. citri. Proteins, including GNA alone, were purified from fermented transformed yeast Pichia pastoris cultures. Recombinant HxTx-Hv1h, HxTx-Hv1h/GNA and GNA were all orally toxic to D. citri, with Day 5 median lethal concentrations (LC50) derived from dose-response artificial diet assays of 27, 20 and 52μM, respectively. Western analysis of whole insect protein extracts confirmed that psyllid mortality was attributable to protein ingestion and that the fusion protein was stable to cleavage by D. citri proteases. When applied topically (either via droplet or spray) HxTx-Hv1h/GNA was the most effective of the proteins causing >70 % mortality 5 days post treatment, some 2 to 3-fold higher levels of mortality as compared to the toxin alone. By contrast, no significant mortality or phenotypic effects were observed for bumble bees (Bombus terrestris L.) fed on the recombinant proteins in acute toxicity assays. This suggests that HxTx-Hv1h/GNA has potential as a novel bioinsecticide for the management of D. citri offering both enhanced target specificity as compared to chemical pesticides and compatibility with integrated pest management (IPM) strategies.

Citation

Miranda, M. P., Fitches, E. C., Sukiran, N. A., Eduardo, W. I., Garcia, R. B., Jaciani, F. J., …Peña, L. (2024). Spider venom neurotoxin based bioinsecticides: A novel bioactive for the control of the Asian citrus psyllid Diaphorina citri (Hemiptera). Toxicon: An Interdisciplinary Journal on the Toxins Derived from Animals, Plants and Microorganisms, 239, Article 107616. https://doi.org/10.1016/j.toxicon.2024.107616

Journal Article Type Article
Acceptance Date Jan 10, 2024
Online Publication Date Jan 12, 2024
Publication Date Feb 23, 2024
Deposit Date Jan 19, 2024
Publicly Available Date Jan 19, 2024
Journal Toxicon
Print ISSN 0041-0101
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 239
Article Number 107616
DOI https://doi.org/10.1016/j.toxicon.2024.107616
Keywords Toxicology
Public URL https://durham-repository.worktribe.com/output/2148772

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