Dr Tim Hawkins t.j.hawkins@durham.ac.uk
Head of Bioimaging
NET4 and RabG3 link actin to the tonoplast and facilitate cytoskeletal remodelling during stomatal immunity
Hawkins, Timothy J.; Kopischke, Michaela; Duckney, Patrick J.; Rybak, Katarzyna; Mentlak, David A.; Kroon, Johan T. M.; Bui, Mai Thu; Richardson, A. Christine; Casey, Mary; Alexander, Agnieszka; De Jaeger, Geert; Kalde, Monika; Moore, Ian; Dagdas, Yasin; Hussey, Patrick J.; Robatzek, Silke
Authors
Michaela Kopischke
Dr Patrick Duckney patrick.duckney@durham.ac.uk
Academic Visitor
Katarzyna Rybak
David A. Mentlak
Dr Johannes Kroon j.t.m.kroon@durham.ac.uk
Senior Experimental Officer
Mai Thu Bui
A. Christine Richardson
Mary Casey
Agnieszka Alexander
Geert De Jaeger
Monika Kalde
Ian Moore
Yasin Dagdas
Professor Patrick Hussey p.j.hussey@durham.ac.uk
Professor
Silke Robatzek
Abstract
Members of the NETWORKED (NET) family are involved in actin-membrane interactions. Here we show that two members of the NET family, NET4A and NET4B, are essential for normal guard cell actin reorganization, which is a process critical for stomatal closure in plant immunity. NET4 proteins interact with F-actin and with members of the Rab7 GTPase RABG3 family through two distinct domains, allowing for simultaneous localization to actin filaments and the tonoplast. NET4 proteins interact with GTP-bound, active RABG3 members, suggesting their function being downstream effectors. We also show that RABG3b is critical for stomatal closure induced by microbial patterns. Taken together, we conclude that the actin cytoskeletal remodelling during stomatal closure involves a molecular link between actin filaments and the tonoplast, which is mediated by the NET4-RABG3b interaction. We propose that stomatal closure to microbial patterns involves the coordinated action of immune-triggered osmotic changes and actin cytoskeletal remodelling likely driving compact vacuolar morphologies.
Citation
Hawkins, T. J., Kopischke, M., Duckney, P. J., Rybak, K., Mentlak, D. A., Kroon, J. T. M., …Robatzek, S. (2023). NET4 and RabG3 link actin to the tonoplast and facilitate cytoskeletal remodelling during stomatal immunity. Nature Communications, 14(1), Article 5848. https://doi.org/10.1038/s41467-023-41337-z
Journal Article Type | Article |
---|---|
Acceptance Date | Aug 29, 2023 |
Online Publication Date | Sep 20, 2023 |
Publication Date | 2023 |
Deposit Date | Sep 26, 2023 |
Publicly Available Date | Sep 27, 2023 |
Journal | Nature Communications |
Publisher | Nature Research |
Peer Reviewed | Peer Reviewed |
Volume | 14 |
Issue | 1 |
Article Number | 5848 |
DOI | https://doi.org/10.1038/s41467-023-41337-z |
Public URL | https://durham-repository.worktribe.com/output/1748706 |
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