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NET4 and RabG3 link actin to the tonoplast and facilitate cytoskeletal remodelling during stomatal immunity

Hawkins, Timothy J.; Kopischke, Michaela; Duckney, Patrick J.; Rybak, Katarzyna; Mentlak, David A.; Kroon, Johan T. M.; Bui, Mai Thu; Richardson, A. Christine; Casey, Mary; Alexander, Agnieszka; De Jaeger, Geert; Kalde, Monika; Moore, Ian; Dagdas, Yasin; Hussey, Patrick J.; Robatzek, Silke

NET4 and RabG3 link actin to the tonoplast and facilitate cytoskeletal remodelling during stomatal immunity Thumbnail


Authors

Michaela Kopischke

Katarzyna Rybak

David A. Mentlak

Mai Thu Bui

A. Christine Richardson

Mary Casey

Agnieszka Alexander

Geert De Jaeger

Monika Kalde

Ian Moore

Yasin Dagdas

Silke Robatzek



Abstract

Members of the NETWORKED (NET) family are involved in actin-membrane interactions. Here we show that two members of the NET family, NET4A and NET4B, are essential for normal guard cell actin reorganization, which is a process critical for stomatal closure in plant immunity. NET4 proteins interact with F-actin and with members of the Rab7 GTPase RABG3 family through two distinct domains, allowing for simultaneous localization to actin filaments and the tonoplast. NET4 proteins interact with GTP-bound, active RABG3 members, suggesting their function being downstream effectors. We also show that RABG3b is critical for stomatal closure induced by microbial patterns. Taken together, we conclude that the actin cytoskeletal remodelling during stomatal closure involves a molecular link between actin filaments and the tonoplast, which is mediated by the NET4-RABG3b interaction. We propose that stomatal closure to microbial patterns involves the coordinated action of immune-triggered osmotic changes and actin cytoskeletal remodelling likely driving compact vacuolar morphologies.

Citation

Hawkins, T. J., Kopischke, M., Duckney, P. J., Rybak, K., Mentlak, D. A., Kroon, J. T. M., …Robatzek, S. (2023). NET4 and RabG3 link actin to the tonoplast and facilitate cytoskeletal remodelling during stomatal immunity. Nature Communications, 14(1), Article 5848. https://doi.org/10.1038/s41467-023-41337-z

Journal Article Type Article
Acceptance Date Aug 29, 2023
Online Publication Date Sep 20, 2023
Publication Date 2023
Deposit Date Sep 26, 2023
Publicly Available Date Sep 27, 2023
Journal Nature Communications
Publisher Nature Research
Peer Reviewed Peer Reviewed
Volume 14
Issue 1
Article Number 5848
DOI https://doi.org/10.1038/s41467-023-41337-z
Public URL https://durham-repository.worktribe.com/output/1748706

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Licence
http://creativecommons.org/licenses/by/4.0/

Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/

Copyright Statement
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.






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