1‐Azathioxanthone Appended Lanthanide(III) DO3A Complexes That Luminesce Following Excitation at 405 nm**

Gundorff Nielsen, Lea; Ravnsborg Hansen, Anne Katrine; Stachelek, Patrycja; Pal, Robert; Just Sørensen, Thomas

Abstract

Since the pioneering report by Selvin, we have been fascinated by the potential of using lanthanide luminescence in bioimaging. The uniquely narrow emission lines and long luminescence lifetimes both provide the potential for background free images together with full certainty of probe localization. General use of lanthanide based bioimaging was first challenged by low brightness, and later by the need of UV (<405 nm) excitation sources not present in commercial microscopes. Here, we designed three lanthanide-based imaging probes based on a known motif to investigate the limitations of 405 nm excitation. These were synthesized, characterized, investigated on dedicated as well as commercial microscopes, and the photophysics was explored in detail. It was proven without doubt that the lanthanide complexes enter the cells and luminesce internally. Even so, no lanthanide luminescence were recovered on the commercial microscopes. Thus, we returned to the photophysical properties that afforded the conclusion that – despite the advances in light sources and photodetectors – we need new designs that can give us brighter lanthanide complexes before bioimaging with lanthanide luminescence becomes something that is readily done.

Citation

Gundorff Nielsen, L., Ravnsborg Hansen, A. K., Stachelek, P., Pal, R., & Just Sørensen, T. (2023). 1‐Azathioxanthone Appended Lanthanide(III) DO3A Complexes That Luminesce Following Excitation at 405 nm**. European Journal of Inorganic Chemistry, 26(24), Article e202300245. https://doi.org/10.1002/ejic.202300245

• Journal Article Type: Article
• Acceptance Date: Jun 14, 2023
• Online Publication Date: Jul 3, 2023
• Publication Date: Aug 23, 2023
• Deposit Date: Sep 14, 2023
• Publicly Available Date: Jul 4, 2024
• Journal: European Journal of Inorganic Chemistry
• Print ISSN: 1434-1948
• Electronic ISSN: 1099-0682
• Publisher: Wiley-VCH Verlag
• Peer Reviewed: Peer Reviewed
• Volume: 26
• Issue: 24
• Article Number: e202300245
• DOI: https://doi.org/10.1002/ejic.202300245
• Keywords: Inorganic Chemistry
• Public URL: https://durham-repository.worktribe.com/output/1737339
• Additional Information: Received: 2023-04-28; Published: 2023-07-03