Dr Abbie Kelly abigail.kelly@durham.ac.uk
Technical Manager
Dr Abbie Kelly abigail.kelly@durham.ac.uk
Technical Manager
Samuel Went samuel.c.went@durham.ac.uk
Post Doctoral Research Associate
Giuseppina Mariano
Dr Liam Shaw liam.p.shaw@durham.ac.uk
Academic Visitor
David Picton david.m.picton@durham.ac.uk
PGR Student Doctor of Philosophy
Samuel Duffner samuel.duffner@durham.ac.uk
PGR Student Master of Science
Isabel Coates
Ryan Herdman-Grant
Julia Gordeeva
Alena Drobiazko
Artem Isaev
Yan-Jiun Lee
Yvette Luyten
Richard D. Morgan
Peter Weigele
Konstantin Severinov
Nicolas Wenner
Jay C.D. Hinton
Professor Tim Blower timothy.blower@durham.ac.uk
Professor
Bacteriophages (phages) outnumber bacteria ten-to-one and cause infections at a rate of 1025 per second. The ability of phages to reduce bacterial populations makes them attractive alternative antibacterials for use in combating the rise in antimicrobial resistance. This effort may be hindered due to bacterial defenses such as Bacteriophage Exclusion (BREX) that have arisen from the constant evolutionary battle between bacteria and phages. For phages to be widely accepted as therapeutics in Western medicine, more must be understood about bacteria–phage interactions and the outcomes of bacterial phage defense. Here, we present the annotated genomes of 12 novel bacteriophage species isolated from water sources in Durham, UK, during undergraduate practical classes. The collection includes diverse species from across known phylogenetic groups. Comparative analyses of two novel phages from the collection suggest they may be founding members of a new genus. Using this Durham phage collection, we determined that particular BREX defense systems were likely to confer a varied degree of resistance against an invading phage. We concluded that the number of BREX target motifs encoded in the phage genome was not proportional to the degree of susceptibility.
Kelly, A., Went, S. C., Mariano, G., Shaw, L. P., Picton, D. M., Duffner, S. J., Coates, I., Herdman-Grant, R., Gordeeva, J., Drobiazko, A., Isaev, A., Lee, Y.-J., Luyten, Y., Morgan, R. D., Weigele, P., Severinov, K., Wenner, N., Hinton, J. C., & Blower, T. R. (2023). Diverse Durham collection phages demonstrate complex BREX defence responses. Applied and Environmental Microbiology, 89(9), Article e00623-23. https://doi.org/10.1128/aem.00623-23
Journal Article Type | Article |
---|---|
Acceptance Date | Jul 10, 2023 |
Online Publication Date | Sep 28, 2023 |
Publication Date | 2023-09 |
Deposit Date | Aug 15, 2023 |
Publicly Available Date | Sep 6, 2023 |
Journal | Applied and Environmental Microbiology |
Print ISSN | 0099-2240 |
Electronic ISSN | 1098-5336 |
Publisher | American Society for Microbiology |
Peer Reviewed | Peer Reviewed |
Volume | 89 |
Issue | 9 |
Article Number | e00623-23 |
DOI | https://doi.org/10.1128/aem.00623-23 |
Public URL | https://durham-repository.worktribe.com/output/1718330 |
Accepted Journal Article
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Copyright Statement
This accepted manuscript is licensed under the Creative Commons Attribution 4.0 licence. https://creativecommons.org/licenses/by/4.0/
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