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Assessment of Toxoplasma gondii lytic cycle and the impact of a gene deletion using 3D label-free optical diffraction holotomography

Koutsogiannis, Zisis; Mina, John G. M.; Suman, Rakesh; Denny, Paul William

Authors

Zisis Koutsogiannis

John G. M. Mina

Rakesh Suman



Abstract

Toxoplasma gondii is a widespread single-celled intracellular eukaryotic apicomplexan protozoan parasite primarily associated with mammalian foetal impairment and miscarriage, including in humans. Is estimated that approximately one third of the human population worldwide is infected by this parasite. Here we used cutting-edge, label-free 3D quantitative optical diffraction holotomography to capture and evaluate the Toxoplasma lytic cycle (invasion, proliferation and egress) in real-time based on the refractive index distribution. In addition, we used this technology to analyse an engineered CRISPR-Cas9 Toxoplasma mutant to reveal differences in cellular physical properties when compared to the parental line. Collectively, these data support the use of holotomography as a powerful tool for the study of protozoan parasites and their interactions with their host cells.

Citation

Koutsogiannis, Z., Mina, J. G. M., Suman, R., & Denny, P. W. (2023). Assessment of Toxoplasma gondii lytic cycle and the impact of a gene deletion using 3D label-free optical diffraction holotomography. Frontiers in Cellular and Infection Microbiology, 13, Article 1237594. https://doi.org/10.3389/fcimb.2023.1237594

Journal Article Type Article
Acceptance Date Jul 17, 2023
Online Publication Date Aug 2, 2023
Publication Date 2023
Deposit Date Aug 3, 2023
Publicly Available Date Aug 3, 2023
Journal Frontiers in Cellular and Infection Microbiology
Electronic ISSN 2235-2988
Publisher Frontiers Media
Peer Reviewed Peer Reviewed
Volume 13
Article Number 1237594
DOI https://doi.org/10.3389/fcimb.2023.1237594
Keywords Infectious Diseases; Microbiology (medical); Immunology; Microbiology
Public URL https://durham-repository.worktribe.com/output/1711732

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Copyright Statement
© 2023 Koutsogiannis, Mina, Suman and
Denny. This is an open-access article
distributed under the terms of the Creative
Commons Attribution License (CC BY). The
use, distribution or reproduction in other
forums is permitted, provided the original
author(s) and the copyright owner(s) are
credited and that the original publication in
this journal is cited, in accordance with
accepted academic practice. No use,
distribution or reproduction is permitted
which does not comply with these terms.





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