TRIPHOSPHORYLATION REACTION

Hodgson, David

Abstract

The disclosure provides a method of producing a triphosphate compound. The method comprises (a) dissolving imidazole in an aqueous alkaline solution to make an imidazole solution; (b) dissolving phosphoryl halide in an organic solvent to make an organic solution; (c) contacting the imidazole solution and the organic solution to produce a solution comprising phosphorylated imidazole (PIm); (d) removing the organic solvent from the solution comprising PIm; and (e) contacting the PIm with a mutant of nucleoside diphosphate kinase (NDPK) and a diphosphate compound, and thereby producing a triphosphate compound.

• Online Publication Date: Jul 5, 2019
• Publication Date: 2020
• Deposit Date: Mar 26, 2020
• Public URL: https://durham-repository.worktribe.com/output/1698482
• Additional Information: Patent URL: https://patentscope.wipo.int/search/en/detail.jsf?docId=WO2020008209&_cid=P22-K8RIXV-59316-1