Fatty acid synthesis in developing leaves of Brassica napus in relation to leaf growth and changes in activity of 3-oxoacyl-ACP reductase

O'Hara, P.; Slabas, AR.; Fawcett, T.

Abstract

In young expanding leaves of Brassica napus, the demand for fatty acids is met by de novo biosynthesis of fatty acid synthase components, as demonstrated by 3-oxoacyl-ACP reductase. Using a novel radio-chemical assay for 3-oxoacyl-ACP reductase and specific antibodies, we have demonstrated a direct relationship between the increase in activity and synthesis of polypeptide. The maximum rate of fatty acid synthesis was between 4 and 7 days post-emergence, but slowed after this point even though 3-oxoacyl-ACP reductase activity was high. Leaf area continued to expand in a linear fashion after reductions in both enzyme activity and the rate of fatty acid synthesis.

Citation

O'Hara, P., Slabas, A., & Fawcett, T. (2001). Fatty acid synthesis in developing leaves of Brassica napus in relation to leaf growth and changes in activity of 3-oxoacyl-ACP reductase. FEBS Letters, 488(1-2), 18-22. https://doi.org/10.1016/s0014-5793%2800%2902406-6

• Journal Article Type: Article
• Publication Date: Jan 1, 2001
• Deposit Date: May 17, 2007
• Journal: FEBS Letters
• Print ISSN: 0014-5793
• Publisher: Federation of European Biochemical Societies
• Peer Reviewed: Peer Reviewed
• Volume: 488
• Issue: 1-2
• Pages: 18-22
• DOI: https://doi.org/10.1016/s0014-5793%2800%2902406-6
• Keywords: 3-Oxoacyl-ACP reductase, Fatty acid synthase, [3H]NADPH, Brassica napus.