Fatty acid synthesis in developing leaves of Brassica napus in relation to leaf growth and changes in activity of 3-oxoacyl-ACP reductase
O'Hara, P.; Slabas, AR.; Fawcett, T.
Professor Tony Fawcett email@example.com
Pro Vice-Chancellor (Education) (Interim)
In young expanding leaves of Brassica napus, the demand for fatty acids is met by de novo biosynthesis of fatty acid synthase components, as demonstrated by 3-oxoacyl-ACP reductase. Using a novel radio-chemical assay for 3-oxoacyl-ACP reductase and specific antibodies, we have demonstrated a direct relationship between the increase in activity and synthesis of polypeptide. The maximum rate of fatty acid synthesis was between 4 and 7 days post-emergence, but slowed after this point even though 3-oxoacyl-ACP reductase activity was high. Leaf area continued to expand in a linear fashion after reductions in both enzyme activity and the rate of fatty acid synthesis.
O'Hara, P., Slabas, A., & Fawcett, T. (2001). Fatty acid synthesis in developing leaves of Brassica napus in relation to leaf growth and changes in activity of 3-oxoacyl-ACP reductase. FEBS Letters, 488(1-2), 18-22. https://doi.org/10.1016/s0014-5793%2800%2902406-6
|Journal Article Type||Article|
|Publication Date||Jan 1, 2001|
|Deposit Date||May 17, 2007|
|Publisher||Federation of European Biochemical Societies|
|Peer Reviewed||Peer Reviewed|
|Keywords||3-Oxoacyl-ACP reductase, Fatty acid synthase, [3H]NADPH, Brassica napus.|
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