Arabidopsis NAP1 is essential for Arp2/3-dependent trichome morphogenesis

Deeks, M.J.; Kaloriti, D.; Davies, B.; Malho, R.; Hussey, P.J.

Abstract

The dynamic nature of the eukaryotic actin cytoskeleton is essential for the locomotion of animal cells and the morphogenesis of plant and fungal cells. The F-actin nucleating/branching activity of the Arp2/3 complex is a key function for all of these processes. The SCAR/WAVE family represents a group of Arp2/3 activators that are associated with lamellipodia formation [1 and 2]. A protein complex of PIR121, NAP1, ABI, and HSPC300 is required for SCAR regulation by cell signaling pathways [3], but the exact nature of this interaction is controversial and represents a continually evolving model [4]. The mechanism originally proposed was of a SCAR trans repressing complex supported by evidence from in vitro experiments [3]. This model was reinforced by genetic studies in the Drosophila central nervous system [5] and Dictyostelium [6], where the knockout of certain SCAR-complex components leads to excessive SCAR-mediated actin polymerization. Conflicting data have steadily accumulated from animal tissue culture experiments suggesting that the complex activates rather than represses in vivo SCAR activity [7, 8 and 9]. Recent biochemical evidence supports the SCAR-complex activator model [9]. Here, we show that genetic observations in Arabidopsis are compatible with an activation model and provide one potential mechanism for the regulation of the newly identified Arabidopsis Arp2/3 complex.

Citation

Deeks, M., Kaloriti, D., Davies, B., Malho, R., & Hussey, P. (2004). Arabidopsis NAP1 is essential for Arp2/3-dependent trichome morphogenesis. Current Biology, 14(15), 1410-1414. https://doi.org/10.1016/j.cub.2004.06.065

• Journal Article Type: Article
• Publication Date: Aug 1, 2004
• Deposit Date: May 16, 2007
• Journal: Current Biology
• Print ISSN: 0960-9822
• Electronic ISSN: 1879-0445
• Publisher: Cell Press
• Peer Reviewed: Peer Reviewed
• Volume: 14
• Issue: 15
• Pages: 1410-1414
• DOI: https://doi.org/10.1016/j.cub.2004.06.065
• Public URL: https://durham-repository.worktribe.com/output/1585034