Y. Bessho
A tRNA aminoacylation system for non-natural amino acids based on a programmable ribozyme
Bessho, Y.; Hodgson, D.R.W.; Suga, H.
Abstract
The ability to recognize tRNA identities is essential to the function of the genetic coding system. In translation aminoacyl-tRNA synthetases (ARSs) recognize the identities of tRNAs and charge them with their cognate amino acids. We show that an in vitro−evolved ribozyme can also discriminate between specific tRNAs, and can transfer amino acids to the 3' ends of cognate tRNAs. The ribozyme interacts with both the CCA-3' terminus and the anticodon loop of tRNAfMet, and its tRNA specificity is controlled by these interactions. This feature allows us to program the selectivity of the ribozyme toward specific tRNAs, and therefore to tailor effective aminoacyl-transfer catalysts. This method potentially provides a means of generating aminoacyl tRNAs that are charged with non-natural amino acids, which could be incorporated into proteins through cell-free translation.
Citation
Bessho, Y., Hodgson, D., & Suga, H. (2002). A tRNA aminoacylation system for non-natural amino acids based on a programmable ribozyme. Nature Biotechnology, 20(7), 723-728. https://doi.org/10.1038/nbt0702-723
Journal Article Type | Article |
---|---|
Publication Date | 2002 |
Deposit Date | Apr 12, 2007 |
Journal | Nature Biotechnology |
Print ISSN | 1087-0156 |
Electronic ISSN | 1546-1696 |
Publisher | Nature Research |
Peer Reviewed | Peer Reviewed |
Volume | 20 |
Issue | 7 |
Pages | 723-728 |
DOI | https://doi.org/10.1038/nbt0702-723 |
Public URL | https://durham-repository.worktribe.com/output/1583423 |
You might also like
Allophycocyanin A is a carbon dioxide receptor in the cyanobacterial phycobilisome
(2022)
Journal Article
Reactivities of electrophilic N–F fluorinating reagents
(2020)
Journal Article
Ubiquitin is a carbon dioxide-binding protein
(2021)
Journal Article
Kinetics of electrophilic fluorination of steroids and epimerisation of fluorosteroids
(2020)
Journal Article
Downloadable Citations
About Durham Research Online (DRO)
Administrator e-mail: dro.admin@durham.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2024
Advanced Search