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Recommendation for measuring and reporting chloride by ISEs in undiluted serum, plasma or blood.

Ben Rayana, M.C.; Burnett, R.W.; Covington, A.K.; D'Orazio, P.; Fogh-Andersen, N.; Jacobs, E.; Kataky, R.; Kulpmann, W.R.; Kuwa, K.; Larsson, L.; Lewenstam, A.; Maas, A.H.J.; Mager, G.; Naskalski, J.W.; Okorodudu, A.O.; Ritter, C.; St John, A.


M.C. Ben Rayana

R.W. Burnett

A.K. Covington

P. D'Orazio

N. Fogh-Andersen

E. Jacobs

W.R. Kulpmann

K. Kuwa

L. Larsson

A. Lewenstam

A.H.J. Maas

G. Mager

J.W. Naskalski

A.O. Okorodudu

C. Ritter

A. St John


The proposed recommendation for measuring and reporting chloride in undiluted plasma or blood by ion-selective electrodes (ISEs) will provide results that are identical to chloride concentrations measured by coulometry for standardized normal plasma or blood samples. It is applicable to all current ISEs dedicated to chloride measurement in undiluted samples that meet the requirements. However, in samples with reduced water concentration, results by coulometry are lower than by ion-selective electrode due to volume displacement. The quantity measured by this standardized ISE procedure is called the ionized chloride concentration. It may be clinically more relevant than the chloride concentration as determined by coulometry, photometry or by ISE after dilution of the sample.


Ben Rayana, M., Burnett, R., Covington, A., D'Orazio, P., Fogh-Andersen, N., Jacobs, E., …St John, A. (2006). Recommendation for measuring and reporting chloride by ISEs in undiluted serum, plasma or blood. Clinical Chemistry and Laboratory Medicine, 44(3), 346-352.

Journal Article Type Article
Publication Date 2006-03
Journal Clinical Chemistry and Laboratory Medicine
Print ISSN 1434-6621
Electronic ISSN 1437-4331
Publisher De Gruyter
Peer Reviewed Peer Reviewed
Volume 44
Issue 3
Pages 346-352
Keywords Blood, Chloride (ionized and total), Coulometry, Ion-selective electrode, Plasma, Standardization.
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