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Cloning and characterization of a Drosophila adenylyl cyclase homologous to mammalian type IX.

Iourgenko, V.; Kliot, B.; Cann, M.J.; Levin, L.R.

Authors

V. Iourgenko

B. Kliot

L.R. Levin



Abstract

A novel Drosophila adenylyl cyclase (AC) was identified by PCR using degenerate primers specific for the known metazoan ACs. The full-length cDNA predicts a protein displaying significant sequence homology with mammalian Type IX AC (AC9). The abundance and size of the message for the Drosophila AC9 homolog (DAC9) changes through development. Biochemical analysis of DAC9 confirms it encodes a functional enzyme which can be activated by forskolin or G protein. Together with the Drosophila Type I AC homolog encoded by the learning and memory gene, rutabaga, the molecular identification of DAC9 demonstrates there is a family of Drosophila AC isoforms reflecting at least part of the diversity of mammalian AC isoforms.

Citation

Iourgenko, V., Kliot, B., Cann, M., & Levin, L. (1997). Cloning and characterization of a Drosophila adenylyl cyclase homologous to mammalian type IX. FEBS Letters, 413(11), 104-108. https://doi.org/10.1016/s0014-5793%2897%2900891-0

Journal Article Type Article
Publication Date 1997-08
Journal FEBS Letters
Print ISSN 0014-5793
Publisher Federation of European Biochemical Societies
Peer Reviewed Peer Reviewed
Volume 413
Issue 11
Pages 104-108
DOI https://doi.org/10.1016/s0014-5793%2897%2900891-0
Keywords Drosophila, Adenylyl cyclase, cAMP, Forskolin, G protein, Signal transduction.
Public URL https://durham-repository.worktribe.com/output/1558846