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Pathogen elicitor-induced changes in the maize extracellular matrix proteome

Chivasa, S; Simon, WJ; Yu, XL; Yalpani, N; Slabas, AR


WJ Simon


N Yalpani

AR Slabas


The extracellular matrix is a vital compartment in plants with a prominent role in defence against pathogen attack. Using a maize cell suspension culture system and pathogen elicitors, responses to pathogen attack that are localised to the extracellular matrix were examined by a proteomic approach. Elicitor treatment of cell cultures induced a rapid change in the phosphorylation status of extracellular peroxidases, the apparent disappearance of a putative extracellular beta-N-acetylglucosamonidase, and accumulation of a secreted putative xylanase inhibitor protein. Onset of the defence response was attended by an accumulation of glyceraldehyde-3-phosphate dehydrogenase and a fragment of a putative heat shock protein. Several distinct spots of both proteins, which preferentially accumulated in cell wall protein fractions, were identified. These three novel observations, viz. (i) secretion of a new class of putative enzyme inhibitor, (ii) the apparent recruitment of classical cytosolic proteins into the cell wall and (ii) the change in phosphorylation status of extracellular matrix proteins, suggest that the extracellular matrix plays a complex role in defence. We discuss the role of the extracellular matrix in signal modulation during pathogen-induced defence responses.


Chivasa, S., Simon, W., Yu, X., Yalpani, N., & Slabas, A. (2005). Pathogen elicitor-induced changes in the maize extracellular matrix proteome. Proteomics, 5(18), 4894-4904.

Journal Article Type Article
Publication Date Dec 1, 2005
Deposit Date Jan 18, 2008
Journal Proteomics
Print ISSN 1615-9853
Electronic ISSN 1615-9861
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 5
Issue 18
Pages 4894-4904
Keywords Dehydrogenase, Extracellular matrix, Glyceraldehyde-3-phosphate, Heat shock protein-82, Maize, Phosphotyrosine.