FGF-2 Release from the Lens Capsule by MMP-2 Maintains Lens Epithelial Cell Viability

Tholozan, F.M.; Gribbon, C.; Li, Z.; Goldberg, M.W.; Prescott, A.R.; McKie, N.; Quinlan, R.A.

Abstract

The lens is an avascular tissue, separated from the aqueous and vitreous humors by its own extracellular matrix, the lens capsule. Here we demonstrate that the lens capsule is a source of essential survival factors for lens epithelial cells. Primary and immortalized lens epithelial cells survive in low levels of serum and are resistant to staurosporine-induced apoptosis when they remain in contact with the lens capsule. Physical contact with the capsule is required for maximal resistance to stress. The lens capsule is also a source of soluble factors including fibroblast growth factor 2 (FGF-2) and perlecan, an extracellular matrix component that enhances FGF-2 activity. Matrix metalloproteinase 2 (MMP-2) inhibition as well as MMP-2 pretreatment of lens capsules greatly reduced the protective effect of the lens capsule, although this could be largely reversed by the addition of either conditioned medium or recombinant FGF-2. These data suggest that FGF-2 release from the lens capsule by MMP-2 is essential to lens epithelial cell viability and survival.

Citation

Tholozan, F., Gribbon, C., Li, Z., Goldberg, M., Prescott, A., McKie, N., & Quinlan, R. (2007). FGF-2 Release from the Lens Capsule by MMP-2 Maintains Lens Epithelial Cell Viability. Molecular Biology of the Cell, 18(11), 4222-4231. https://doi.org/10.1091/mbc.e06-05-0416

• Journal Article Type: Article
• Publication Date: Aug 1, 2007
• Deposit Date: Oct 1, 2008
• Publicly Available Date: Oct 1, 2008
• Journal: Molecular biology of the cell
• Electronic ISSN: 1939-4586
• Publisher: American Society for Cell Biology
• Peer Reviewed: Peer Reviewed
• Volume: 18
• Issue: 11
• Pages: 4222-4231
• DOI: https://doi.org/10.1091/mbc.e06-05-0416
• Public URL: https://durham-repository.worktribe.com/output/1557624
• Publisher URL: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17699594