Evaluation of cataract formation in fish exposed to environmental radiation at Chernobyl and Fukushima

Lerebours, Adélaïde; Regini, Justyn; Quinlan, Roy A.; Wada, Toshihiro; Pierscionek, Barbara; Devonshire, Martin; Kalligeraki, Alexia A.; Uwineza, Alice; Young, Laura; Girkin, John M.; Warwick, Phil; Smith, Kurt; Hoshino, Masato; Uesugi, Kentaro; Yagi, Naoto; Terrill, Nick; Shebanova, Olga; Snow, Tim; Smith, Jim T.

Abstract

Recent studies apparently finding deleterious effects of radiation exposure on cataract formation in birds and voles living near Chernobyl represent a major challenge to current radiation protection regulations. This study conducted an integrated assessment of radiation exposure on cataractogenesis using the most advanced technologies available to assess the cataract status of lenses extracted from fish caught at both Chernobyl in Ukraine and Fukushima in Japan. It was hypothesised that these novel data would reveal positive correlations between radiation dose and early indicators of cataract formation.

The structure, function and optical properties of lenses were analysed from atomic to millimetre length scales. We measured the short-range order of the lens crystallin proteins using Small Angle X-Ray Scattering (SAXS) at both the SPring-8 and DIAMOND synchrotrons, the profile of the graded refractive index generated by these proteins, the epithelial cell density and organisation and finally the focal length of each lens.

The results showed no evidence of a difference between the focal length, the epithelial cell densities, the refractive indices, the interference functions and the short-range order of crystallin proteins (X-ray diffraction patterns) in lens from fish exposed to different radiation doses. It could be argued that animals in the natural environment which developed cataract would be more likely, for example, to suffer predation leading to survivor bias. But the cross-length scale study presented here, by evaluating small scale molecular and cellular changes in the lens (pre-cataract formation) significantly mitigates against this issue.

Citation

Lerebours, A., Regini, J., Quinlan, R. A., Wada, T., Pierscionek, B., Devonshire, M., …Smith, J. T. (2023). Evaluation of cataract formation in fish exposed to environmental radiation at Chernobyl and Fukushima. Science of the Total Environment, 902, Article 165957. https://doi.org/10.1016/j.scitotenv.2023.165957

• Journal Article Type: Article
• Acceptance Date: Jul 30, 2023
• Online Publication Date: Aug 13, 2023
• Publication Date: Dec 1, 2023
• Deposit Date: Jan 17, 2024
• Publicly Available Date: Jan 17, 2024
• Journal: Science of The Total Environment
• Print ISSN: 0048-9697
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 902
• Article Number: 165957
• DOI: https://doi.org/10.1016/j.scitotenv.2023.165957
• Keywords: Pollution; Waste Management and Disposal; Environmental Chemistry; Environmental Engineering
• Public URL: https://durham-repository.worktribe.com/output/2148802